ROLE OF EICOSANOIDS IN ABORTION AND ITS PREVENTION BY TREATMENT WITH FLUNIXIN MEGLUMINE IN COWS DURING THE 1ST TRIMESTER OF PREGNANCY
ROLE OF EICOSANOIDS IN ABORTION AND ITS PREVENTION BY TREATMENT WITH FLUNIXIN MEGLUMINE IN COWS DURING THE 1ST TRIMESTER OF PREGNANCY
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DOI：
10.1111/j.1439-0442.1991.tb01034.x
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发表时间：
1991-07-01
期刊：
影响因子：
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通讯作者：
OSBURN, BI
中科院分区：
文献类型：
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作者：
GIRI, SN;STABENFELDT, GH;OSBURN, BI
以4.16 ng/kg/min的速度静脉滴注大肠杆菌内毒素6小时以上(总剂量1.5-mU/kg)，其中3头奶牛在妊娠60-72小时内以4.16 ng/kg/min的速度静脉滴注60-72小时内流产。流产奶牛的血浆PGF2-α水平在1小时显著升高至零时间对照(ZTC)的289%，并在9小时内保持较高水平。在非流产奶牛中，除2小时外，PGF2-α水平未受影响。流产和未流产奶牛的血浆TxB2水平在6小时内都比ZTC对照组升高了6~18倍。6-keto-PGF1-α水平仅在流产奶牛中显著升高2~3倍。流产奶牛的血浆皮质醇水平在5小时内最高增加到ZTC的1,500%。此后，这一水平逐渐下降，但在24小时内仍显著升高。未流产奶牛皮质醇水平在5小时时仅为ZTC的280%，12小时时恢复到ZTC水平。流产奶牛的血浆孕酮水平在12小时前不受影响，然后在18小时后逐渐下降，在3、4和5天时降至极低水平。输注内毒素可引起流产奶牛和非流产奶牛的高血糖和流产奶牛的乳酸酸血症。静脉注射内毒素前1小时和注射后13小时分别给予环氧合酶抑制剂氟尼辛葡甲胺1.1 mg/kg，可完全阻止内毒素诱导的流产及血浆中PGF2-α、TxB2和6-keto-PGF1-α浓度的升高。前列腺素E水平未受影响。虽然FM治疗不能消除内毒素引起的血浆皮质醇和乳酸水平的升高，但它有效地阻止了孕酮的显著下降和血糖浓度的上升。结论：FM在预防妊娠1s孕期细菌感染引起的内毒素引起的牛流产方面具有良好的疗效。
Intravenous infusion of E. coli endotoxin at a rate of 4.16 ng/kg/min over 6 hr (total dose 1.5-mu-g/kg) in 5 cows in the first trimester of gestation induced abortion between 60 and 72 hr in three cows. Plasma PGF2-alpha levels in the aborting cows increased significantly to 289% of the zero time control (ZTC) at 1 hr and remained elevated for 9 hr. The PGF2-alpha level remained unaffected in the non-aborting cows except at 2 hr. The plasma TxB2 levels were increased by 6 to 18 fold for 6 hr in both the aborting and non-aborting cows relative to their ZTC controls. The 6-keto-PGF1-alpha levels were significantly increased to 2 to 3 fold only in the aborting cows. Plasma cortisol levels were increased maximally to 1,500% of ZTC at 5 hr in the aborting cows. Thereafter, the levels gradually declined but remained significantly elevated for 24 hr. The increases in the cortisol levels in the non-aborting cows were only 280% of ZTC at 5 hr and returned to ZTC value by 12 hr. Plasma progesterone levels in the aborting cows remained unaffected until 12 hr followed by a progressive decline through 18 hr to extremely low levels at 3, 4, and 5 days. Endotoxin-infusion caused hyperglycemia in both aborting and non-aborting cows and lactic acidemia in the aborting cows. Treatment with two doses of flunixin meglumine (FM, 1.1 mg/kg), an inhibitor of cyclooxygenase, 1 hr prior to endotoxin infusion and then 13 hr later, completely prevented the endotoxin-induced abortion and increases in the plasma PGF2-alpha, TxB2 and 6-keto-PGF1-alpha concentrations. The PGE level remained unaffected. Although FM treatment failed to abolish endotoxin-induced increases in the plasma cortisol and lactic acid levels, it effectively prevented marked decreases in the progesterone and increases in the glucose concentrations. It was concluded that the use of FM offers therapeutic promise in preventing bovine abortion caused by endotoxin resulting from bacterial infection during the 1s trimester of gestation.