Luminescent lanthanide chelate contrast agents and detection of lesions in the hamster oral cancer model
Luminescent lanthanide chelate contrast agents and detection of lesions in the hamster oral cancer model
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DOI：
10.1366/000370203769699063
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发表时间：
2003-10-01
影响因子：
3.5
通讯作者：
Motamedi, M
中科院分区：
文献类型：
--
作者：
Bornhop, DJ;Griffin, JMM;Motamedi, M
稀土络合物是一类独特的分子，由于其极大的斯托克斯位移和长的荧光寿命，在生物医学领域已被证明是有用的。这些分子在低本底或零本底区域产生荧光的能力使这类分子成为生物环境中广泛应用的造影剂的极佳候选者。在这里，我们介绍了一种基于1，4，7，10-四氮杂环十二烷大环(Cyclen)的新型Tb螯合物造影剂的制备、光谱表征和应用，用于检测叙利亚仓鼠颊囊的早期恶性病变。TB-P(CTMB)在低光子通量的紫外光激发下发出明亮的蓝绿色发光。作为一项初步研究，采用DMBA处理的金黄地鼠颊囊上皮癌模型，并使用TB-P(CTMB)作为局部药物用于病变组织的视觉检测。在这项初步研究中，该试剂倾向于与早期恶性病变有关，表明TB-P(CTMB)可用作造影剂，帮助识别早期口腔癌病变。
Lanthanide chelates are a somewhat unique class of molecules that have proven to be useful in the biomedical field due to their extremely large Stokes' shift and long fluorescent lifetimes. The ability of these molecules to produce fluorescence in the low- or zero-background regime makes this class of molecules excellent candidates for use as contrast agents for a wide variety of applications in biological settings. Here we present the preparation, spectroscopic characterization, and application of a new terbium chelate contrast agent, based on the 1,4,7,10-tetraazacyclododecane macrocycle (cyclen), for detection of early-stage malignant lesions in the Syrian hamster cheek pouch. Tb-P(CTMB) delivers bright blue-green luminescence when excited with low photon fluxes of UV light. As a pilot study, the DMBA-treated Golden Hamster Cheek pouch epithelial cancer model was employed and Tb-P(CTMB) was used as a topical agent for the visual detection of diseased tissue. In this preliminary study the agent tended to associate with early-stage malignant lesions, suggesting that Tb-P(CTMB) could be used as a contrast agent to aid in identifying early-stage oral cancer lesions.