Digital DNA typing at a second hypervariable locus by minisatellite variant repeat mapping.
Digital DNA typing at a second hypervariable locus by minisatellite variant repeat mapping.
复制标题
通过小卫星变异重复定位对第二个高变位点进行数字 DNA 分型。
DOI：
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发表时间：
1993
影响因子：
3.5
通讯作者：
A. Jeffreys
中科院分区：
文献类型：
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作者：
D. L. Neil;A. Jeffreys
通过 PCR (MVR-PCR) 进行的小卫星变异重复单元作图可测定变异重复单元沿小卫星等位基因的散布模式。绘制高度多态性小卫星 MS31A（D7S21 位点）中的此类内部变异图谱，揭示了等位基因变异性的极端水平，远远超过等位基因长度分析可检测到的水平。侧翼碱基取代多态性使得大量 MS31A 等位基因的 5' 结构能够通过等位基因特异性 MVR-PCR 从基因组 DNA 中衍生出来。目前已绘制出 100 多个等位基因图谱，而且全部都不同。几个等位基因显示出相关的内部结构，其中一些提供了等位基因变异极性的证据，让人想起在其他两个高变小卫星 D1S8 (MS32) 和 D16S309 (MS205) 中看到的极性。我们还描述了 MS31A 的二倍体数字编码，包括通过双工 MVR-PCR 对 MS31A 和第二个基因座 MS32 进行同时编码，这极大地增强了该技术的潜在法医应用。
Minisatellite variant repeat unit mapping by PCR (MVR-PCR) assays the interspersion pattern of variant repeat units along minisatellite alleles. Mapping such internal variation in the highly polymorphic minisatellite MS31A (locus D7S21), reveals extreme levels of allelic variability, far in excess of that detectable by allele length analysis. Flanking base substitutional polymorphisms have enabled the 5' structure of large numbers of MS31A alleles to be derived from genomic DNA by allele-specific MVR-PCR. More than 100 alleles have now been mapped and all are different. Several alleles show related internal structures and some of these provide evidence of polarity in allelic variation reminiscent of that seen at two other hypervariable minisatellites, D1S8 (MS32) and D16S309 (MS205). We also describe the diploid digital coding of MS31A, including the simultaneous coding of MS31A and a second locus, MS32, by duplex MVR-PCR, which greatly enhances the potential forensic applications of this technique.