Analysis of Phospholipids, Lysophospholipids, and Their Linked Fatty Acyl Chains in Yellow Lupin Seeds (Lupinus luteus L.) by Liquid Chromatography and Tandem Mass Spectrometry
Analysis of Phospholipids, Lysophospholipids, and Their Linked Fatty Acyl Chains in Yellow Lupin Seeds (Lupinus luteus L.) by Liquid Chromatography and Tandem Mass Spectrometry
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DOI：
10.3390/molecules25040805
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发表时间：
2020-02-02
期刊：
影响因子：
4.6
通讯作者：
Cataldi, Tommaso R., I
中科院分区：
文献类型：
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作者：
Calvano, Cosima Damiana;Bianco, Mariachiara;Cataldi, Tommaso R., I
采用亲水相互作用液相色谱（HILIC）和电喷雾离子化（ESI）-傅里叶变换（FT）轨道阱或线性离子阱串联质谱（LIT-MS/MS）对黄羽扇豆种子磷脂质组进行了表征。磷脂酰胆碱（PC）是最丰富的种类（41.6%），其次是溶血型LPC（30 ± 11%），磷脂酰乙醇胺（PE，13 ± 4%），磷脂酰甘油（PG，5.1 +/- 1.7%）、磷脂酸（PA，4.9 +/- 1.8%）、磷脂酰肌醇（PI，4.7 +/- 1.1%）和LPE（1.2 ± 0.5%）。通过在负离子模式下观察到的明确的碎片模式推断了几种LPC和LPE的两种异构形式的发生。报道了200多种极性脂质的前所未有的表征，包括52种PC，42种PE，42种PA，35种PG，16种LPC，13种LPE和10种PI。PL中最丰富的脂肪酸（FA）作为酯化酰基链是18：1（油酸），18：2（亚油酸），16：0（棕榈酸），和18：3（亚麻酸）与相对较高的长脂肪酰基链，如22：0（二十二烷酸），24：0（二十四烷酸），20：1（gondoic），和22：1（芥酸）。通过在100 ℃酸性条件下对化学水解样品浸提液进行45 min的反相（RP）LC-ESI-FTMS分析，证实了其存在。
Hydrophilic interaction liquid chromatography (HILIC) and electrospray ionization (ESI) coupled to either Fourier-transform (FT) orbital-trap or linear ion-trap tandem mass spectrometry (LIT-MS/MS) was used to characterize the phospholipidome of yellow lupin (Lupinus luteus) seeds. Phosphatidylcholines (PC) were the most abundant species (41 6%), which were followed by lyso-forms LPC (30 +/- 11%), phosphatidylethanolamines (PE, 13 +/- 4%), phosphatidylglycerols (PG, 5.1 +/- 1.7%), phosphatidic acids (PA, 4.9 +/- 1.8%), phosphatidylinositols (PI, 4.7 +/- 1.1%), and LPE (1.2 0.5%). The occurrence of both isomeric forms of several LPC and LPE was inferred by a well-defined fragmentation pattern observed in negative ion mode. An unprecedented characterization of more than 200 polar lipids including 52 PC, 42 PE, 42 PA, 35 PG, 16 LPC, 13 LPE, and 10 PI, is reported. The most abundant fatty acids (FA) as esterified acyl chains in PL were 18:1 (oleic), 18:2 (linoleic), 16:0 (palmitic), and 18:3 (linolenic) with relatively high contents of long fatty acyl chains such as 22:0 (behenic), 24:0 (lignoceric), 20:1 (gondoic), and 22:1 (erucic). Their occurrence was confirmed by reversed-phase (RP) LC-ESI-FTMS analysis of a chemically hydrolyzed sample extract in acid conditions at 100 degrees C for 45 min.