Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines
Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines
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DOI：
10.1111/aab.12532
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发表时间：
2019-09-01
影响因子：
2.6
通讯作者：
Stojkovic, Dejan
中科院分区：
文献类型：
--
作者：
Aleksic, Milena;Stanisavljevic, Danijela;Stojkovic, Dejan
嘧霉胺是一种人工合成的杀菌剂，广泛用于处理不同作物的采前和采后真菌病害，研究了其对不同真菌菌株的抗真菌活性。以黑曲霉为模型生物，研究了嘧霉胺对真菌麦角甾醇的影响。此外，还研究了嘧霉胺是否能有效减少樱桃番茄受害果实中的曲霉菌腐烂现象。评价了杀菌剂对不同人细胞株的细胞毒作用。此外，还研究了其对细胞增殖的影响。黑曲霉是对嘧霉胺抗性最强的真菌菌株(MFC 1.88亩/亩L-1)。麦角甾醇的加入使黑曲霉的MFC增加了一倍，表明该化合物可能干扰真菌细胞膜的主要甾醇麦角甾醇。在0.75 mg/伤口的嘧霉胺处理下，未检测到黑曲霉在樱桃番茄果实中的发病率。在50 ng/mU的L-1剂量下，嘧霉胺对人细胞株已有一定的细胞毒作用，而150 ng/mU的L-1作用后，细胞增殖标记物Ki67的表达下降。这些结果表明，嘧霉胺在减少各种真菌病原体方面是有效的，但由于其细胞毒性，该杀菌剂的进一步使用应重新评估。
Pyrimethanil, a synthetic fungicide widely used for the treatment of pre- and postharvest fungal diseases on different agricultural crops, was explored for its antifungal activity on different fungal strains. The effect of pyrimethanil on fungal ergosterol was tested by using Aspergillus niger as a model organism. Furthermore, it was investigated, if pyrimethanil can effectively reduce the appearance of Aspergillus rot in wounded cherry tomato fruits. The fungicide cytotoxic effect on different human cell lines was evaluated. In addition, its influence on cell proliferation was studied. A. niger was the most resistant fungal strain (MFC 1.88 mu g mu L-1) to the effect of pyrimethanil. Addition of ergosterol doubled the MFC on A. niger, indicating that the compound might interfere with ergosterol, main sterol of fungal cell membrane. Disease incidence of A. niger in wounded cherry tomato fruits was not detected with pyrimethanil treatment of 0.75 mg/wound. Some cytotoxic effects of pyrimethanil on human cell lines were recorded already at 50 ng mu L-1, while the expression of Ki67 marker of proliferation was decreased with 150 ng mu L-1. These results altogether indicate that pyrimethanil is effective in reducing various fungal pathogens, but further use of this fungicide should be reevaluated because of its cytotoxicity.