Synergistic stimulation of prolactin release by phorbol ester, A23187 and forskolin.
Synergistic stimulation of prolactin release by phorbol ester, A23187 and forskolin.
复制标题
佛波酯、A23187 和毛喉素协同刺激催乳素释放。
DOI：
10.1016/0006-291x(84)90291-2
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发表时间：
1984
影响因子：
3.1
通讯作者：
Rasmussen,H
中科院分区：
文献类型：
--
作者：
Delbeke,D;Kojima,I;Dannies,PS;Rasmussen,H
比较 12-0-十四烷酰佛波醇-13-乙酸酯 (TPA)、A23187、for-skolin 和促甲状腺素释放激素 (TRH) 对 GH4C1 细胞催乳素释放的影响。 TPA 导致 2 倍释放，6 分钟或更长时间后达到最大值，并持续 30 分钟或更长时间。 A23187 仅引起较小且可变的反应，并在 4 至 6 分钟内达到峰值。 TPA 和 A23187 的组合导致释放量快速增加 3 至 5 倍，然后缓慢下降。 TRH 使催乳素释放增加 3 至 5 倍，在 4 分钟内达到最大值，然后以较低速率持续释放。 Forskolin 本身几乎没有作用，但 TPA 和 A23187 组合或 TRH 会增强释放。这些数据与Ca2+信使系统的两个分支参与TRH的作用的模型一致，即钙调蛋白分支和C激酶分支相互作用导致大量持续释放。 Forskolin 通过调节细胞的环 AMP 含量来确定 Ca2+ 信使系统运行的设定点。
The effects of 12-0-tetradecanoyl-phorbol-13-acetate (TPA), A23187, for-skolin and thyrotropin-releasing hormone (TRH) on prolactin release from GH4C1cells were compared. TPA caused a 2-fold release, maximum after 6 or more min, that was sustained for 30 min or more. A23187 caused only a small and variable response that peaked within 4 to 6 min. Combination of TPA and A23187 caused a rapid 3- to 5-fold increase in release that declined slowly. TRH increased prolactin release 3- to 5-fold, reaching a maximum within 4 min, followed by sustained release at lower rates. Forskolin had little effect by itself, but potentiated release caused either by combined TPA and A23187, or by TRH. These data are consistent with a model in which two branches of the Ca2+messenger system participate in the action of TRH, a calmodulin branch and a C-kinase branch that interact to cause large amounts of sustained release. Forskolin, by regulating the cyclic AMP content of the cell determines the set point around which the Ca2+messenger system operates.