Antagonistic control of oxidative stress-induced cell death in Arabidopsis by two related, plant-specific zinc finger proteins
Antagonistic control of oxidative stress-induced cell death in Arabidopsis by two related, plant-specific zinc finger proteins
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DOI：
10.1073/pnas.1130421100
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发表时间：
2003-05-27
影响因子：
11.1
通讯作者：
Dangl, JL
中科院分区：
文献类型：
--
作者：
Epple, P;Mack, AA;Dangl, JL
植物细胞程序性死亡最常见的形式是与成功的植物免疫反应相关的超敏反应(HR)。在HR之前，会发生氧化猝发，同时产生活性氧中间体(ROI)和NO。拟南芥LSD1基因编码植物细胞程序性死亡的负调控因子，满足病原菌响应过程中与ROI管理相关的过程调控的几个标准。在这里，我们证明了一个高度保守的LSD1类似物LOL1，作为细胞死亡的正向调节因子。操纵LOL1的表达改变了Isd1植物的超氧化物依赖、失控的细胞死亡表型和正常的HR。我们还表明，LSD1和LOL1对铜锌超氧化物歧化酶的积累具有拮抗作用，这与通过维持ROI动态平衡来控制细胞死亡的功能一致。
The most familiar form of plant programmed cell death is the hypersensitive response (HR) associated with successful plant immune responses. HR is preceded by an oxidative burst and the generation of both reactive oxygen intermediates (ROI) and NO. The Arabidopsis LSD1 gene encodes a negative regulator of plant programmed cell death that meets several criteria for a regulator of processes relevant to ROI management during pathogen responses. Here we demonstrate that a highly conserved LSD1 paralogue, LOL1, acts as a positive regulator of cell death. Manipulation of LOL1 expression alters both the superoxide-dependent, runaway cell death phenotype of Isd1 plants and the normal HR. We also show that LSD1 and LOL1 have antagonistic effects on copper-zinc superoxide dismutase accumulation, consistent with functions in cell death control via maintenance of ROI homeostasis.