On-column ligand/receptor derivatization coupled to affinity capillary electrophoresis.
On-column ligand/receptor derivatization coupled to affinity capillary electrophoresis.
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柱上配体/受体衍生化与亲和毛细管电泳相结合。
DOI：
10.1007/978-1-59745-376-9_26
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Gomez,FrankA
中科院分区：
文献类型：
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作者：
Zavaleta,Jose;Chinchilla,Dinora;Gomez,Alvaro;Silverio,Catherine;Azad,Maryam;Gomez,FrankA
小分子的柱上衍生与亲和毛细管电泳的联用是近5年才实现的。在该技术中，将试剂和配体或受体的多个区域注入毛细管柱中。电泳时，样品区重叠，产生产物。继续电泳导致产物与受体（或配体，如果受体被衍生化）重叠，从而导致所述化合物的迁移时间偏移。随后的斯卡查德分析使用非相互作用的标准实现了结合常数。本文中，我们描述了使用柱上配体和受体衍生化偶联部分填充ACE（PFACE）来探测来自东方链霉菌的万古霉素（货车）和来自放线菌的替考拉宁（Teic）与D-Ala-D-Ala末端肽的结合。
The coupling of on-column derivatization of small molecules to affinity capillary electrophoresis (ACE) has only been realized during the past 5 yr. In this technique, multiple zones of reagent(s) and ligand or receptor are injected into the capillary column. Upon electrophoresis, zones of sample overlap, yielding product. Continued electrophoresis results in the product overlapping with receptor (or ligand, if the receptor was derivatized), thereby causing a shift in migration time of the compound in question. Subsequent Scatchard analysis using noninteracting standards realizes a binding constant. Herein, we describe the use of on-column-ligand and receptor derivatization coupled to partial-filling ACE (PFACE) to probe the binding of vancomycin (Van) fromStreptomyces orientalisand teicoplanin (Teic) fromActinoplanes teicomyceticusto D-Ala-D-Ala terminus peptides.