Toxic effect of NiCl2 on development of the bursa of Fabricius in broiler chickens.
Toxic effect of NiCl2 on development of the bursa of Fabricius in broiler chickens.
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DOI：
10.18632/oncotarget.6591
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发表时间：
2016-01-05
期刊：
影响因子：
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通讯作者：
Guo H
中科院分区：
文献类型：
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作者：
Yin S;Cui H;Peng X;Fang J;Zuo Z;Deng J;Wang X;Wu B;Guo H
本试验采用实验病理学、流式细胞术（FCM）和实时荧光定量聚合酶链反应（qRT-PCR）等方法，研究了在饲粮中添加0、300、600和900 mg/kg氯化镍（NiCl2） 42 d后，氯化镍对肉鸡法氏囊发育的毒性影响。结果表明：饲粮中添加300 mg/kg的NiCl2对法氏囊发育有过致毒性抑制作用，表现为淋巴细胞组织病理学和相对重量降低，G0/G1期（延长的不分裂状态）增加，S期（DNA复制）和增殖指数降低，凋亡细胞百分比增加。同时，bax、细胞色素c （cyt c）、凋亡肽酶激活因子1 （Apaf-1）、caspase-3、caspase-6、caspase-7、caspase-9 mRNA表达水平升高，bcl-2 mRNA表达水平降低。毒抑制法氏囊发育最终导致肉鸡体液免疫功能受损，导致B淋巴细胞数量和B淋巴细胞活性降低。本研究为进一步研究Ni及Ni复合物对b细胞或法氏囊的作用机制提供了新的依据。
This study was conducted with objective of evaluating the toxic effects of nickel chloride (NiCl2) on development of bursa of Fabricius in broilers fed on diets supplemented with 0, 300, 600 and 900 mg/kg of NiCl2 for 42 days by using the methods of experimental pathology, flow cytometry (FCM), and quantitative real-time polymerase chain reaction (qRT-PCR). The results showed that dietary NiCl2 in 300 mg/kg and over induced toxic suppression in the bursal development, which was characterized by decreasing lymphocytes histopathologically and relative weight, increasing G0/G1 phase (a prolonged nondividing state), reducing S phase (DNA replication) and proliferating index, and increasing percentages of apoptotic cells. Concurrently, the mRNA expression levels of bax, cytochrome c (cyt c), apoptotic peptidase activating factor 1 (Apaf-1), caspase-3, caspase-6, caspase-7 and caspase-9 were increased and the bcl-2 mRNA expression levels were decreased. The toxic suppression of bursal development finally impaired humoral immunity duo to the reduction of B lymphocyte population and B lymphocyte activity in the broiler chicken. This study provides new evidences for further studying the effect mechanism of Ni and Ni compoundson B-cell or bursa of Fabricius.