Optimization of Cellulase Immobilization with Sodium Alginate-Polyethylene for Enhancement of Enzymatic Hydrolysis of Microcrystalline Cellulose Using Response Surface Methodology
Optimization of Cellulase Immobilization with Sodium Alginate-Polyethylene for Enhancement of Enzymatic Hydrolysis of Microcrystalline Cellulose Using Response Surface Methodology
复制标题
响应面法优化海藻酸钠-聚乙烯固定化纤维素酶强化微晶纤维素酶的酶解
DOI：
10.1007/s12010-021-03517-9
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发表时间：
2021-02-05
影响因子：
3
通讯作者：
Liu, Yanping
中科院分区：
文献类型：
--
作者：
Guo, Rongxin;Zheng, Xusheng;Liu, Yanping
采用海藻酸钠-聚乙二醇（SA-PEG）固定化纤维素酶，使纤维素酶可重复使用。利用傅立叶变换红外光谱和扫描电镜对固定化纤维素酶的基质进行了检测和表征。与SA固定化纤维素酶相比，相对酶活和固定化率分别提高了25%和18%。固定化酶在温度和pH下的应用范围比游离酶大，热稳定性提高。将固定化酶应用于以MCC为底物的酶解反应中。最佳酶解条件为：SA-PEG固定化纤维素酶的用量为3.55 g/g底物总固形物，底物浓度为13.16%，pH为5.11。与第一轮水解相比，SA-PEG固定化纤维素酶水解MCC的还原糖得率提高了133%。在重复使用5个循环后，当MCC被SA-PEG-固定化纤维素酶水解时，还原糖的总收率与游离纤维素酶的总收率相似。与游离酶相比，固定化酶的产率最高为22.68%。因此，固定化纤维素酶具有较高的酶解性能。
A novel method of immobilizing cellulase on sodium alginate (SA)-polyethylene glycol (PEG) enabled the cellulase to be used repeatedly. The matrix of the immobilized cellulase was detected and characterized using Fourier transform infrared spectroscopy and scanning electron microscopy. In comparison with SA-immobilized cellulase, the relative enzyme activity and immobilization rate increased by 25% and 18%, respectively. The application range of the immobilized enzyme in terms of temperature and pH was larger than that of the free enzyme, and its thermal stability increased. The immobilized enzyme was used in enzymatic hydrolysis, in which MCC was used as the substrate. The optimal conditions for enzymatic hydrolysis were as follows: the dosage of SA-PEG-immobilized cellulase was 3.55 g/g total solids of the substrate, the concentration of the substrate was 13.16%, and the pH was 5.11. In comparison with the yield of reducing sugars in the first round of hydrolysis of MCC by SA-immobilized cellulase, the yield in the hydrolysis of MCC by SA-PEG-immobilized cellulase increased by 133%. After five cycles of repeated use, the total yield of reducing sugars when MCC was hydrolyzed by SA-PEG-immobilized cellulase was similar to that achieved with free cellulase. In comparison with the free enzyme, the highest yield when the immobilized enzyme was used was 22.68%. Therefore, the immobilized cellulase exhibited high performance in enzymatic hydrolysis.