Relationship of phosphorylation potential and oxygen consumption in isolated perfused rat hearts.
Relationship of phosphorylation potential and oxygen consumption in isolated perfused rat hearts.
复制标题
离体灌注大鼠心脏磷酸化电位与耗氧量的关系。
DOI：
10.1016/0022-2828(80)90058-9
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发表时间：
1980
影响因子：
5
通讯作者：
H. Kammermeier
中科院分区：
文献类型：
--
作者：
J. Giesen;H. Kammermeier
在分离的线粒体中（状态4至3），呼吸作用线性依赖于培养基的磷酸化电位（log [ATP]/[ADP][Pi]）。进行实验以在完整心肌中寻找类似的关系。对代谢物结合或区室化进行以下校正，以获得相应代谢物的肌浆浓度：（1）根据文献校正常数和比例值。(2)将ATP和Pi校正为（1）假设pH恒定，根据CPK平衡计算ADP。(3)校正为（2）根据文献假设细胞溶质pH值的呼吸依赖性变化。(4)根据文献计算与H+分布相关的Pi分布。对于（1）至（4），获得了增加的相关系数（0.43至0.95），表明在完整心脏中，线粒体呼吸以与线粒体悬浮液中相同的方式通过磷酸化电位或间接地由于CPK平衡通过[CrP]·[H+]· K CPK [Cr]·[Pi]进行调节。
In isolated mitochondria (State 4 to 3) respiration depends linearly on the phosphorylation potential (log [ATP]/[ADP][Pi] of the medium. Experiments were performed to look for similar relationship in the intact myocardium. The following corrections for metabolite binding or compartmentation were carried out to obtain sarcoplasmic concentrations of the respective metabolites:(1) Correction for constant and proportional values according to literature.(2) Correction for ATP and Pi as (1) calculation of ADP from CPK equilibrium assuming a constant pH.(3) Correction as (2) assuming respiration dependent changes of cytosolic pH according to literature.(4) Calculation of Pi distribution correlated to H+ distribution according to literature. For (1) to (4) increasing coefficients of correlation (0.43 to 0.95) were obtained indicating that in the intact heart mitochondrial respiration is regulated in the same way as in mitochondrial suspensions by the phosphorylation potential or indirectly because of CPK equilibrium by the ratio [CrP]·[H+]· K CPK [Cr]·[Pi].