Pradimicin-IRD from Amycolatopsis sp. IRD-009 and its antimicrobial and cytotoxic activities
Pradimicin-IRD from Amycolatopsis sp. IRD-009 and its antimicrobial and cytotoxic activities
复制标题
DOI：
10.1080/14786419.2018.1434639
复制
发表时间：
2019-06-18
影响因子：
2.2
通讯作者：
Moraes, Luiz A. B.
中科院分区：
文献类型：
--
作者：
Bauermeister, Anelize;Calil, Felipe A.;Moraes, Luiz A. B.
从巴西热带雨林恢复区土壤中分离到一种新的多环抗生素——pradimicin-IRD。这种分子是固体培养基中产生的主要化合物。新化合物采用前体离子聚焦法（高效液相色谱-串联质谱联用）进行检测，该方法可用于鉴定异常氨基糖基。该化合物已被分离，并通过高分辨率质谱、一维和二维核磁共振实验对其结构进行了鉴定。Pradimicin-IRD对无乳链球菌（MIC为3.1 μ g/mL）、铜绿假单胞菌（MIC为3.1 μ g/mL）和金黄色葡萄球菌（MIC为3.1 μ g/mL）具有潜在的抗菌活性，对肿瘤和非肿瘤细胞系也具有细胞毒性，其IC50值从HCT-116结肠癌细胞的0.8 μ M到MM 200黑色素瘤细胞的2.7 μ M不等。特别是，这些生物特性是首次在该化学类别中被描述出来。
A new polycyclic antibiotic, pradimicin-IRD, was isolated from actinobacteria Amycolatopsis sp. IRD-009 recovered from soil of Brazilian rainforest undergoing restoration area. This molecule is the major compound produced in solid culture media. The new compound was detected by a focused method of precursor ion (high-performance liquid chromatography coupled to tandem mass spectrometer) developed previously to identify unusual aminoglycosyl sugar moieties. The compound was isolated and its structure was, therefore, elucidated by high-resolution mass spectrometry, and 1D and 2D nuclear magnetic resonance experiments. Pradimicin-IRD displayed potential antimicrobial activity against Streptococcus agalactiae (MIC 3.1 mu g/mL), Pseudomonas aeruginosa (MIC 3.1 mu g/mL) and Staphylococcus aureus (MIC 3.1 mu g/mL), and also cytotoxicity against tumour and non-tumour cell lines with IC50 values ranging from 0.8 mu M in HCT-116 colon carcinoma cells to 2.7 mu M in MM 200 melanoma cells. Particularly, these biological properties are described for the first time for this chemical class.[GRAPHICS].