3D T cell motility in jammed microgels
3D T cell motility in jammed microgels
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DOI：
10.1088/1361-6463/aae813
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发表时间：
2019-01-09
影响因子：
3.4
通讯作者：
Angelini, Thomas E.
中科院分区：
文献类型：
--
作者：
Bhattacharjee, Tapomoy;Angelini, Thomas E.
对细胞在3D空间中的体外行为的探索揭示了细胞的形态、细胞所产生的力以及细胞内机械机械的结构与2D不同，表明了在3D空间进行研究的重要性。然而，当前的3D方法使用连续的聚合物网络来研究3D中的细胞，这提出了与实现细胞运动的瞬时孔隙空间的需求以及控制3D细胞分布的复杂性相关的众多挑战。为了克服这些挑战，我们开发了一种由堵塞的微凝胶制成的3D培养介质；堵塞的微凝胶在液体细胞生长介质中膨胀的流变性允许细胞分散到具有明确结构和材料特性的3D环境中。在这项工作中，我们探索了在不同屈服应力下制备的密闭微凝胶生长介质体系中T细胞的运动性，找到了与微凝胶之间的孔隙空间相关的时间尺度和长度-尺度依赖的动力学。堵塞的微凝胶代表了一类具有独特性质的软物质，尚未被用于研究细胞在3D空间中的运动或力学；我们通过堵塞的微凝胶对细胞运动的观察展示了T细胞如何在多孔环境中导航，并为未来在未知领域的大量研究提供指导。
Exploration of in vitro cell behavior in 3D space has revealed that cell morphology, cell generated forces, and the architecture of the intracellular mechanical machinery differ from their 2D counterparts, demonstrating the importance of investigation in 3D. However, current 3D methods employ continuous polymer networks to study cells in 3D which present numerous challenges associated with the need for transient pore-space to enable cell motion and the complexities of controlling the 3D cell distribution. To overcome these challenges, we have developed a 3D culture medium made from jammed microgels; the rheological properties of jammed microgels swelled in liquid cell growth media allow the dispersal of cells in into a 3D environment with well-defined structural and material properties. In this work, we explore the motility of T cells in systems of jammed microgel growth media prepared with different yield stresses, finding timescale and length-scale dependent dynamics that are correlated with the pore-space between the microgels. Jammed microgels represent a class of soft matter with unique properties that has not been leveraged for the study of cell motion or mechanics in 3D space; our observations of cell motion through jammed microgels demonstrate how T cells navigate porous environments and provide guidance for a multitude of future investigations in unexplored territory.