Energetics and chronology of phototransients in the light response of the purple membrane of Halobacterium halobium.
Energetics and chronology of phototransients in the light response of the purple membrane of Halobacterium halobium.
复制标题
盐杆菌紫色膜光响应中光瞬变的能量学和年代学。
DOI：
10.1016/0005-2728(76)90021-9
复制
发表时间：
1976
期刊：
影响因子：
--
通讯作者：
S. Caplan
中科院分区：
文献类型：
--
作者：
W. V. Sherman;R. Korenstein;S. Caplan
在0-60 °C温度范围内，测定了紫膜碎片在水和2H_2O中悬浮液中光瞬变的形成和衰减的Arrhenius参数。动力学同位素效应的发现表明，质子转移步骤参与的形成和衰减的两个最长寿命的瞬态吸收在410 nm和660 nm，分别。结果还表明，这些瞬态不占据紫膜内的细菌视紫红质的自发去兴奋的单一途径。紫膜在25-30 °C的水和2 H2O中均发生相转变。
Arrhenius parameters for formation and decay of phototransients in suspensions of purple membrane fragments in H2O and2H2O have been determined in the temperature range 0–60 °C. Kinetic isotope effects are found which show that proton transfer steps are involved in both formation and decay of the two longest-lived transients absorbing at 410 nm and 660 nm, respectively. The results also suggest that these transients do not occupy a single pathway in the spontaneous deexcitation of bacteriorhodopsin within the purple membrane. Purple membrane undergoes a phase transition at 25–30 °C in both H2O and2H2O.