Computational analysis and identification of an emergent human adenovirus pathogen implicated in a respiratory fatality.
Computational analysis and identification of an emergent human adenovirus pathogen implicated in a respiratory fatality.
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DOI：
10.1016/j.virol.2010.10.020
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发表时间：
2011-01-20
期刊：
影响因子：
3.7
通讯作者：
Chodosh J
中科院分区：
文献类型：
--
作者：
Robinson CM;Singh G;Henquell C;Walsh MP;Peigue-Lafeuille H;Seto D;Jones MS;Dyer DW;Chodosh J
腺病毒感染通常是急性的、自限性的，与死亡无关。然而，我们提供了一种在法国分离的新型重组人腺病毒(HAdV-D56)的基因组和生物信息学分析，该病毒导致罕见的新生儿死亡，并导致三名护理新生儿的医护人员患上角结膜炎。全基因组比对揭示了预期的五角体碱基、六邻体、E3和纤维编码区的多样性，并为广泛重组提供了证据。BootScan分析证实了HAdV-D9、HAdV-D26、HAdV-D15和HAdV-D29在五元碱基和六元蛋白质之间的重组，并以推测的蛋白质内的高变环为中心。蛋白质结构分析表明，HAdV-D8、HAdV-D9和HAdV-D53与HAdV-D8、HAdV-D9和HAdV-D53相似，这可能是该病毒嗜眼性的原因。根据这些数据，该病毒似乎是一种新的HAdV-D型(HAdV-D56)，强调了重组事件在人腺病毒进化和新腺病毒病原体出现中的重要性。
Adenoviral infections are typically acute, self-limiting, and not associated with death. However, we present the genomic and bioinformatics analysis of a novel recombinant human adenovirus (HAdV-D56) isolated in France that caused a rare neonatal fatality, and keratoconjunctivitis in three health care workers who cared for the neonate. Whole genome alignments revealed the expected diversity in the penton base, hexon, E3, and fiber coding regions, and provided evidence for extensive recombination. Bootscan analysis confirmed recombination between HAdV-D9, HAdV-D26, HAdV-D15, and HAdV-D29 in the penton base and hexon proteins, centered around hypervariable loops within the putative proteins. Protein structure analysis of the fiber coding region revealed similarity with HAdV-D8, HAdV-D9, and HAdV-D53, possibly accounting for the ocular tropism of the virus. Based on these data, this virus appears to be a new HAdV-D type (HAdV-D56), underscoring the importance of recombination events in human adenovirus evolution and the emergence of new adenovirus pathogens.