Expression of local hepatocyte growth factor system in vascular tissues.
Expression of local hepatocyte growth factor system in vascular tissues.
复制标题
血管组织中局部肝细胞生长因子系统的表达。
DOI：
--
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Toshio Ogihara
中科院分区：
文献类型：
--
作者：
Y. Nakamura;R. Morishita;J. Higaki;I. Kida;M. Aoki;A. Moriguchi;Kazuo Yamada;S. Hayashi;Y. Yo;Kunio Matsumoto;Toshikazu Nakamura;Toshio Ogihara
Since endothelial cells (EC) are known to secrete various anti-proliferative and vasodilating factors, an agent that promotes seeding or regeneration of EC may have potential therapeutic value against vascular smooth muscle cell (VSMC) proliferation. For the above purpose, we have found that hepatocyte growth factor (HGF) fulfills such conditions. However, the local HGF system has not yet clarified. Therefore, we investigated endogenous HGF production and its specific receptor (c-met) in vascular tissues. Our results revealed the expressions of HGF and its receptor (c-met) mRNA in endothelial cells, VSMC and neointimal VSMC of rat assessed by RT-PCR. HGF and c-met mRNA were also detected in human aortic endothelial cells and VSMC. Endothelial cells and VSMC of both rat and human produce and secrete immunoreactive HGF as assessed by ELISA. Of importance, the existence of local HGF system (HGF and c-met) was also observed in vivo in intact abdominal aorta of SD and Wistar rats. Overall, this study demonstrated that HGF is expressed and secreted from endothelial cells and VSMC of both rat and human in vitro as well as in vivo. Since HGF has characteristics of an endothelium-specific growth factor, locally synthesized HGF in endothelial cells and VSMC may have a role in vascular functions in autocrine-paracrine manner.
