Alterations in the distribution of histone H3 phosphorylation in mitotic plant chromosomes in response to cold treatment and the protein phosphatase inhibitor cantharidin
Alterations in the distribution of histone H3 phosphorylation in mitotic plant chromosomes in response to cold treatment and the protein phosphatase inhibitor cantharidin
复制标题
DOI：
10.1023/a:1020940313841
复制
发表时间：
2002-01-01
影响因子：
2.6
通讯作者：
Houben, A
中科院分区：
文献类型：
--
作者：
Manzanero, S;Rutten, T;Houben, A
组蛋白H3在Ser10位的磷酸化在植物细胞分裂中的作用尚不清楚。这个时间与染色体凝聚有关，植物减数分裂的研究表明，它参与了姐妹染色单体的凝聚。在有丝分裂中，植物染色体仅在着丝粒周围区域高度磷酸化。为了调节H3的磷酸化，不同植物的根尖分生组织分别用蛋白磷酸酶抑制剂斑泻素和冰水处理。在Ser 10使用针对磷酸化H3的抗体的免疫染色显示，斑泻素处理后，整个有丝分裂染色体上都有高水平的H3磷酸化，这与第一次减数分裂中所见的分布相似。在用冰水处理过的分生组织中分离的染色体中，异染色区和核仁组织者区，以及着丝粒周围区，在H3被高度磷酸化。斑泻素和冰水也影响纺锤体组装和染色体长度，但这些影响似乎与H3磷酸化的变化没有直接联系。
The function of the phosphorylation of histone H3 at Ser 10 in plant cell division is uncertain. The timing correlates with chromosome condensation, and studies in plant meiosis suggest that it is involved in sister chromatid cohesion. In mitosis, plant chromosomes are highly phosphorylated in the pericentromeric region only. In order to modulate H3 phosphorylation, root meristems of different plant species were treated with the protein phosphatase inhibitor cantharidin or with ice-water. Immunostaining using an antibody specific to phosphorylated H3 at Ser 10 revealed a high level of H3 phosphorylation along the whole mitotic chromosome after cantharidin treatment, which resembles the distribution seen exclusively in first meiotic division. In chromosomes that were isolated from meristems treated with ice - water, the heterochromatic regions and nucleolar organizer regions, in addition to the pericentromeric region, were highly phosphorylated at H3. Cantharidin and ice - water also affected spindle assembly and chromosome length, but these effects did not seem to be directly linked to changes in H3 phosphorylation.