Immunoglobulin lambda gene rearrangement can precede kappa gene rearrangement.
Immunoglobulin lambda gene rearrangement can precede kappa gene rearrangement.
复制标题
DOI：
10.1155/1990/56014
复制
发表时间：
1990-01-01
期刊：
影响因子：
--
通讯作者：
Wabl, M
中科院分区：
文献类型：
--
作者：
Berg, J;McDowell, M;Wabl, M
免疫球蛋白基因在B淋巴细胞分化过程中通过连接基因片段而产生。小鼠前B细胞含有功能性免疫球蛋白重链基因，但不含轻链基因。虽然只有一个重链基因座，但有两个轻链基因座：κ和λ。据报道，生殖系结构中的κ基因座从未（在人类中）或很少（在小鼠中）存在于具有功能重排的β链基因的细胞中。已经提出了两种解释来解释这一点：（a）有序重排理论，该理论假定前B细胞中的轻链基因重排首先在κ基因座尝试，并且只有在不能产生功能性κ链时才尝试重排λ基因座;和（B）随机理论，其假定λ基因座处的重排以本质上比κ基因座处的重排慢得多的速率进行。在这里，我们表明，产生的kappa基因座是否已经失去了它的种系排列，结果是兼容的随机理论，无论是否链基因。
Immunoglobulin genes are generated during differentiation of B lymphocytes by joining gene segments. A mouse pre-B cell contains a functional immunoglobulin heavy-chain gene, but no light-chain gene. Although there is only one heavy-chain locus, there are two light-chain loci: kappa and lambda. It has been reported that kappa loci in the germ-line configuration are never (in man) or very rarely (in the mouse) present in cells with functionally rearranged lambda-chain genes. Two explanations have been proposed to explain this: (a) the ordered rearrangement theory, which postulates that light-chain gene rearrangement in the pre-B cell is first attempted at the kappa locus, and that only upon failure to produce a functional kappa chain is there an attempt to rearrange the lambda locus; and (b) the stochastic theory, which postulates that rearrangement at the lambda locus proceeds at a rate that is intrinsically much slower than that at the kappa locus. We show here that lambda-chain genes are generated whether or not the kappa locus has lost its germ-line arrangement, a result that is compatible only with the stochastic theory.