Production and Secretion of hCG and hCG Subunits by Trophoblastic Tissue
Production and Secretion of hCG and hCG Subunits by Trophoblastic Tissue
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滋养层组织产生和分泌 hCG 和 hCG 亚基
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
S. Tojo
中科院分区：
文献类型：
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作者：
Y. Ashitaka;R. Nishimura;M. Takemori;S. Tojo
先进的技术在糖蛋白激素的纯化和鉴定方面取得了巨大的成功。人的糖蛋白激素，如黄体生成素(LH)、卵泡刺激素(FSH)、促甲状腺激素(TSH)和人绒毛膜促性腺激素(HCG)都是由两个不完全相同的α和β亚基衍生的蛋白质核心组成的，支链碳水化合物侧链连接在天冬氨酸、丝氨酸和/或苏氨酸上。与α亚基之间基本相同或几乎相同的氨基酸序列相比，天然激素的生物学特性很大程度上是由β亚基(也带有特定的抗原位点)贡献的，并且在结构上对每个物种都是独特的。针对人绒毛膜促性腺激素β亚基产生的抗血清能较好地区分人促黄体生成素和人绒毛膜促性腺激素，而大多数来自完整激素的抗血清则不能(Amir，1972)。
Advanced techniques have brought great success in purification and characterization of glycoprotein hormones. The human glycoprotein hormones, such as luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and human chorionic gonadotropin (hCG) are all composed of a protein core derived from two non-identical alpha and beta subunits, with branched carbohydrate side chains radically attached to asparagine, serines, and/or threonine. In contrast to the essentially identical or nearly identical amino acid sequence among the α-subunits, the biological properties of native hormones are greatly contributed by the β-subunit (which also carries specific antigenic sites) and are structurally unique for each species. Antisera generated to the β-subunit of hCG discriminate comparatively well between hLH and hCG, while most of those derived from intact hormones do not (Amir, 1972).