Method for resolution and quantification of components of the non-photochemical quenching (qN)
Method for resolution and quantification of components of the non-photochemical quenching (qN)
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DOI：
10.1007/s11120-010-9564-6
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发表时间：
2010-06
影响因子：
3.7
通讯作者：
K. Roháček
中科院分区：
文献类型：
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作者：
K. Roháček
本文介绍了一种新的叶绿素荧光猝灭分析方法，该方法利用实验数据中的多指数函数非线性回归，计算出叶绿素荧光（qN）的非光化学猝灭的（至少）三个分量的值。与“能量”有关的系数公式Chla荧光的（Δ pH依赖的）猝灭（qE）、状态转换猝灭（qT）和光/抑制猝灭（qI）是基于三个假设发现的：（i）qN及其所有分量的暗弛豫动力学具有指数性质，（ii）叠加原理对单个Chla荧光猝灭过程有效;（iii）相同的参考荧光水平（即在暗适应状态下的最大可变Chla荧光产率，FV）用于定义qN及其分量。所有的定义以及算法的分析识别的qN组件的理论澄清和实验测试。所描述的理论结果是一个相当简单的方程，允许计算所有qN分量（qE，qT，qI）的值以及相应淬火过程的弛豫半衰期（τ1/2）。证明了在上述假设下，qN=qE+qT+qI成立.理论推导的方程进行了测试，并讨论了非应力和应力光合有效样品所获得的结果。给出了由实验数据快速估算qN分量值的半经验公式。
A new method of the chlorophyll (Chl)afluorescence quenching analysis is described, which allows the calculation of values of (at least) three components of the non-photochemical quenching of the variable Chlafluorescence (qN) using a non-linear regression of a multi-exponential function within experimental data. Formulae for coefficients of the “energy”-dependent (ΔpH-dependent) quenching (qE), the state-transition quenching (qT) and the photo/inhibitory quenching (qI) of Chlafluorescence were found on the basis of three assumptions: (i) the dark relaxation kinetics ofqN, as well as of all its components, is of an exponential nature, (ii) the superposition principle is valid for individual Chlafluorescence quenching processes and (iii) the same reference fluorescence level (namely the maximum variable Chlafluorescence yield in the dark-adapted state,FV) is used to define bothqNand its components. All definitions as well as the algorithms for analytical recognition of theqNcomponents are theoretically clarified and experimentally tested. The described theory results in a rather simple equation allowing to compute values for allqNcomponents (qE,qT,qI) as well as the half-times of relaxation (τ1/2) of corresponding quenching processes. It is demonstrated that under the above assumptions it holds:qN=qE+qT+qI. The theoretically derived equations are tested, and the results obtained are discussed for non-stressed and stressed photosynthetically active samples. Semi-empirical formulae for a fast estimation of values of theqNcomponents from experimental data are also given.