Speciation of iron coordinated by phytosiderophores by use of HPLC with pulsed amperometric detection and AAS
Speciation of iron coordinated by phytosiderophores by use of HPLC with pulsed amperometric detection and AAS
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DOI：
10.1007/s00216-002-1302-2
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发表时间：
2002-08-01
影响因子：
4.3
通讯作者：
Römheld, V
中科院分区：
文献类型：
--
作者：
Weber, G;Neumann, G;Römheld, V
mugineic酸家族的植物铁载体，和各自的铁物种，已被分离的阴离子交换色谱与NaOH梯度洗脱。两种不同的检测方法并行使用，脉冲安培法（PAD）的植物铁载体和原子吸收光谱法（AAS）的铁。这种组合使得能够鉴定分离的铁物质。在30分钟内分离并检测到多达五种不同的铁-两种不同的植物铁载体，两种氨基酸，和一种尚未被鉴定但最有可能是植物铁载体的分解产物。的检测限是在低μ mol L-1浓度范围内，这是足够低的测定在真实的植物样品，即使稀释后。该方法已被应用于缺铁小麦和大麦植物的根洗液和非缺铁玉米的木质部渗出物。
Phytosiderophores of the mugineic acid family, and the respective iron species, have been separated by anion-exchange chromatography with NaOH gradient elution. Two different detection methods were used in parallel, pulsed amperometry (PAD) for phytosiderophores and atomic absorption spectrometry (AAS) for iron. This combination enables identification of separated iron species. Up to five different iron species were separated and detected within 30 min - two different phytosiderophore species, two amino acid species, and one species which has not yet been identified but which is most probably a decomposition product of phytosiderophores. The detection limit was in the low mumol L-1 concentration range, which is sufficiently low for determination in real plant samples, even after dilution. The method has been applied to root washings of iron-deficient wheat and barley plants and to a xylem exudate of non-deficient maize.