Obstacles and challenges for tissue engineering and regenerative medicine: Australian nuances
Obstacles and challenges for tissue engineering and regenerative medicine: Australian nuances
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DOI：
10.1111/1440-1681.12899
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发表时间：
2018-04-01
影响因子：
2.9
通讯作者：
Grounds, Miranda D.
中科院分区：
文献类型：
--
作者：
Grounds, Miranda D.
介绍了组织工程学在再生医学中应用的临床需求和历史方法，以及澳大利亚在这一领域的研究进展，并讨论了组织培养研究的巨大进展(A部分)。人类干细胞和产生生物支架的新方法的结合为基础生物学和生理学的体外研究、制药工业的药物测试和高通量筛选以及器官和设备的先进组织工程提供了巨大的机会。这里的未来是光明的。使用动物模型和人类在体内应用这些生物工程进展的主要障碍出现(B部分)，首先讨论了活组织的复杂性和临床转移到大规模人类情况所需的规模增加的挑战。虽然无细胞生物支架植入的临床成功(宿主细胞的数量)可能会扩大到人类使用，但主要的挑战涉及(通常)体内(供体或自体)细胞的低存活率，这些细胞在植入活体之前在组织培养中扩增和生长。另一个主要挑战是在人体范围内对植入的组织/器官进行再血管化。组织生物工程的创新方法和快速发展为克服这些主要障碍和扩大这些技术的临床应用带来了巨大的希望。
The clinical need and historical approaches to tissue engineering as applied to regenerative medicine are introduced, along with comments on activities in this field around Australia, and then the huge advances for tissue culture studies are discussed (Part A). Combinations of human stem cells and new approaches for generating bioscaffolds present great opportunities for in vitro studies of basic biology and physiology, drug testing and high throughput screening for the pharmaceutical industry, and the advanced tissue engineering of organs and devices. The future here is bright. The major obstacles arise with in vivo application of these bioengineering advances using animal models and humans (Part B), and the complexity of living tissues and the challenges of increased scale required for clinical translation to the large human situation are first discussed. While clinical success seen with implantation of acellular bioscaffolds (with population by host cells) is likely to expand for human use, the major challenge relates to (generally) low survival in vivo of (donor or autologous) cells that are expanded and grown in tissue culture before implantation into the living body. Another major challenge is revascularisation of implanted tissues/organs at the human scale. The innovative approaches and rapid advances in tissue bioengineering hold great promise for overcoming these major obstacles and extending the clinical applications of these technologies.