Telomerase activity: a prevalent marker of malignant human prostate tissue.
Telomerase activity: a prevalent marker of malignant human prostate tissue.
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DOI：
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发表时间：
1996
期刊：
影响因子：
11.2
通讯作者：
H. Sommerfeld;A. Meeker;Mieczyslaw A. Piatyszek;G. Bova;J. Shay;D. S. Coffey
中科院分区：
文献类型：
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作者：
H. Sommerfeld;A. Meeker;Mieczyslaw A. Piatyszek;G. Bova;J. Shay;D. S. Coffey
我们迫切需要一种生化标志物来检测前列腺的恶性性质和病理状态。我们报告端粒酶活性与前列腺癌有关，但在良性疾病和正常腺体中不存在。因此，端粒酶是一个潜在的前列腺癌诊断标志物。对25例在根治性前列腺切除术时切除的人前列腺进行解剖，以获得匹配的正常、中央区良性前列腺增生（BPH）和病理证实的癌组织的相邻区域。这些匹配的组织样本进行了端粒酶活性检测使用敏感的PCR技术。正常组织均无端粒酶活性。相比之下，25例癌症中有21例（84%）呈强阳性。在乳腺癌切除术时，4个淋巴结转移呈阳性，且均为端粒酶活性强阳性。25例癌旁组织中仅3例（12%）呈弱阳性。在10例单纯因BPH行开放手术的BPH患者中未检测到端粒酶活性。所有五种可用的人前列腺癌细胞系（DU 145、LNCaP、PC 3、PPC 1和大津）均为强阳性。在几种人类癌症中已经观察到短端粒长度。我们还测量了取自9例根治性前列腺切除术的27例正常、BPH和癌组织的端粒长度。癌组织的端粒明显且一致地短于邻近的正常组织或邻近的BPH组织。我们的研究结果表明，端粒酶活性，以及端粒长度，可能是区分前列腺癌与正常和良性前列腺组织的标志物。
We urgently need biochemical markers to detect the malignant nature and pathological states of the human prostate. We report that telomerase activity is associated with prostate cancer but absent in the benign disease and normal gland. Telomerase is, therefore, a potential diagnostic marker for prostate cancer. Twenty-five human prostates resected at the time of radical prostatectomy were dissected to obtain matched adjacent areas of normal, central zone benign prostatic hyperplasia (BPH), and pathologically confirmed cancer tissue. These matched tissue samples were assayed for telomerase activity using a sensitive PCR technique. None of the normal tissues exhibited telomerase activity. In contrast, 21 of the 25 (84%) cancers were strongly positive. At the time of prostatectomy, four lymph nodes were positive for metastases and all were strongly positive for telomerase activity. In adjacent BPH tissues taken from the cancerous prostates, only 3 of the 25 tissues (12%) were weakly positive. Telomerase activity was not detected in ten BPH samples recovered from patients who underwent open surgery solely for BPH. All five available cell lines of human prostate cancer (DU145, LNCaP, PC3, PPC1, and TSU) were strongly positive. Short telomere lengths have been observed in several human cancers. We also measured the telomere lengths in 27 matched samples of normal, BPH, and cancer tissue taken from nine radical prostatectomies. The telomeres from cancer tissue were significantly and consistently shorter than either the adjacent normal or adjacent BPH tissues. Our results indicate that telomerase activity, as well as telomere lengths, may be markers for distinguishing prostate cancer from normal and benign prostate tissues.