The combination between cations and sulfated polysaccharide from abalone gonad (Haliotis discus hannai Ino)
The combination between cations and sulfated polysaccharide from abalone gonad (Haliotis discus hannai Ino)
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阳离子与鲍鱼性腺硫酸化多糖的结合
DOI：
10.1016/j.carbpol.2018.01.100
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发表时间：
2018-05-15
影响因子：
11.2
通讯作者：
Wen, Chengrong
中科院分区：
文献类型：
--
作者：
Guo, Yiming;Cong, Shuang;Wen, Chengrong
采用刚果红试验、x射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描电镜（SEM）等手段研究了阳离子（Na+、K+、Ca2+、Mg2+）对鲍鱼性腺硫酸酸化多糖（AGSP）结构的影响。结果表明，AGSP存在局部有序螺旋构象。阳离子可以通过离子键与AGSP中的阴离子基团结合，使分子链的静电斥力和交联减弱。二价阳离子对AGSP构象的影响比一价阳离子更明显，由于相互作用位点较多，二价阳离子导致AGSP聚集。简而言之，加入阳离子后，AGSP分子链通过离子键交联，二价阳离子通过静电相互作用诱导AGSP聚集。该研究将为进一步研究AGSP构象提供有价值的见解。
Effects of cations (Na+, K+, Ca2+, Mg2+) on the structure of abalone gonad sulfated polysaccharide (AGSP) were studied by means of Congo red test, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM). The results showed that the local ordered helical conformation was observed in AGSP. The cations could combine with anionic groups in AGSP by ionic bonds, which caused the weakening of electrostatic repulsion and crosslinking of molecular chains. Furthermore, the effects of divalent cations on the conformation were more obvious than that of monovalent cations, and divalent cations led to the aggregation of AGSP due to the more interaction sites. In short, AGSP molecular chains were crosslinked through ionic bonds after adding cations, and, divalent cations could induce the aggregation of AGSP by electrostatic interactions. This study will provide valuable insights for the further research on AGSP conformation.