Complement Lysis: the Ultrastructure and Orientation of the C5b‐9 Complex on Target Sheep Erythrocyte Membranes
Complement Lysis: the Ultrastructure and Orientation of the C5b‐9 Complex on Target Sheep Erythrocyte Membranes
复制标题
补体裂解：目标羊红细胞膜上 C5b-9 复合物的超微结构和取向
DOI：
10.1111/j.1365-3083.1978.tb00425.x
复制
发表时间：
1978
影响因子：
3.7
通讯作者：
V. Speth
中科院分区：
文献类型：
--
作者：
Jørgen Tranum;S. Bhakdi;B. Bhakdi;Ole J. Bjerrum;V. Speth
C5b - 9复合物来源于人血清，组装在靶羊红细胞膜上，是一个一端被环包围的薄壁圆柱体。气缸的总高度为150 Å，其中环空贡献了30 Å。圆筒的内径明显均匀，为100 Å。环外径200 Å。补体溶解后在膜上看到的经典补体“环”代表垂直于膜上的C5b‐9圆柱体。薄壁圆筒固定在膜上，环位于外膜糖萼。在C5b - 9配合物的附着位点，膜双分子层的连续性受到干扰，表明存在跨膜孔。这些数据基本上支持补体溶解的“甜甜圈”理论。
The C5b‐9 complex derived from human serum and assembled on target sheep erythrocyte membranes is a thin‐walled cylinder rimmed by an annulus at one end. The total height of the cylinder is 150 Å, towards which the annulus contributes 30 Å. The cylinder has an apparently uniform internal diameter of 100 Å. The external diameter of the annulus is 200 Å. The classical complement ‘rings’ visualized on membranes after complement lysis represent such C5b‐9 cylinders perpendicularly oriented on the membranes. The thin‐walled cylinder is anchored in the membrane maim and the annulus located in the exterior membrane glycocalyx. At the sites of attachment of the C5b‐9 complexes, the continuity of the membrane bilayer is disturbed and the presence of trans‐membrane pores is indicated The data essentially support the ‘doughnut’ theory of complement lysis.