On‐chip chiral and achiral separation of amphetamine and related compounds labeled with 4‐fluoro‐7‐nitrobenzofurazane
On‐chip chiral and achiral separation of amphetamine and related compounds labeled with 4‐fluoro‐7‐nitrobenzofurazane
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4-氟-7-硝基苯并呋喃标记的安非他明及相关化合物的芯片上手性和非手性分离
作者：
S. Wallenborg;I. Lurie;D. Arnold;C. Bailey
安非他明和类似化合物已被标记为4-氟-7-硝基苯并呋咱，并在微型芯片上进行分析。采用胶束电动毛细管色谱（MEKC）和激光诱导荧光（LIF）检测器分离去甲麻黄碱、麻黄碱、卡西酮、伪麻黄碱、甲卡西酮、苯丙胺和甲基苯丙胺。研究了使用中性和带负电荷的环糊精（CD）与和不加有机改性剂和/或十二烷基硫酸钠（SDS）的个别药物的手性分离。使用高度硫酸化的γ-CD（HS-γ-CD）与低浓度SDS组合获得最佳结果。为了获得（+/-）-去甲麻黄碱、（+/-）麻黄碱、（+/-）-伪麻黄碱、（+/-）-甲卡西酮、（+/-）-苯丙胺和（+/-）-甲基苯丙胺的混合物的完全分离，需要向分离缓冲液中加入少量（1.5 mM）SDS。使用S-折叠分离通道，8 kV的分离电压和由50 mM磷酸盐（pH 7.35）、10 mM HS-γ-CD和1.5 mM SDS组成的缓冲液，在7 min内实现优化的手性分离。
Amphetamine and analogous compounds have been labeled with 4‐fluoro‐7‐nitrobenzofurazane and analyzed on a microfabricated chip. Separation of norephedrine, ephedrine, cathinone, pseudoephedrine, methcathinone, amphetamine and methamphetamine is demonstrated using micellar electrokinetic capillary chromatography (MEKC) and laser‐induced fluorescence (LIF) detection. Chiral separations of individual drugs were studied using neutral and negatively charged cyclodextrins (CDs) with and without the addition of an organic modifier and/or sodium dodecyl sulfate (SDS). The best results were obtained using a highly sulfated γ‐CD (HS‐γ‐CD) in combination with a low concentration of SDS. To obtain complete separation of a mixture of (+/−)‐norephedrine, (+/−)ephedrine, (+/−)‐pseudoephedrine, (+/−)‐methcathinone, (+/−)‐amphetamine and (+/−)‐methamphetamine it was necessary to add a small amount (1.5 mM) of SDS to the separation buffer. Optimized chiral separation was achieved within 7 min using an S‐folded separation channel, a separation voltage of 8 kV and a buffer consisting of 50 mM phosphate (pH 7.35), 10 mM HS‐γ‐CD and 1.5 mM SDS.