StHAB1, a negative regulatory factor in abscisic acid signaling, plays crucial roles in potato drought tolerance and shoot branching
StHAB1, a negative regulatory factor in abscisic acid signaling, plays crucial roles in potato drought tolerance and shoot branching
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DOI：
10.1093/jxb/erad292
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发表时间：
2023-11-21
影响因子：
6.9
通讯作者：
Song, Botao
中科院分区：
文献类型：
--
作者：
Liu, Tengfei;Dong, Liepeng;Song, Botao
脱落酸（阿坝）在植物耐旱性和生长中起着重要作用。A组蛋白2C型磷酸酶（PP 2Cs）是阿坝信号传导和植物适应逆境的负调节因子。关于马铃薯A组PP 2C的功能的知识是有限的。在这里，我们报告说，马铃薯A组PP 2C StHAB 1广泛表达在马铃薯植物和强烈诱导阿坝和干旱。StHAB 1的抑制增强了马铃薯阿坝敏感性和耐旱性，而显性突变体StHAB 1（G276 D）的过表达损害了阿坝敏感性和耐旱性。StHAB 1与几乎所有阿坝受体和Snf 1相关激酶OST 1相互作用。抑制StHAB 1和过表达StHAB 1（G276 D）改变马铃薯生长形态;值得注意的是，StHAB 1（G276 D）的过表达导致过度的枝条分枝。RNA测序分析表明，生长素外排载体基因StPIN 3，StPIN 5和StPIN 8在StHAB 1（G276 D）过表达腋芽中上调。相应地，生长素浓度在StHAB 1（G276 D）过表达腋芽中降低，这与生长素抑制侧分支生长的作用一致。BRANCHED 1 s（StBRC 1a和StBRC 1b）的表达在StHAB 1（G276 D）过表达腋芽中没有变化，表明StHAB 1（G276 D）过表达不会通过调节BRC 1表达引起腋芽生长。研究结果表明，StHAB 1在马铃薯耐旱性和分枝发育中起重要作用，StHAB 1是脱落酸信号通路中的负调控因子，通过影响气孔开放和生长素运输来调节马铃薯耐旱性和分枝发育。
Abscisic acid (ABA) is critical in drought tolerance and plant growth. Group A protein type 2C phosphatases (PP2Cs) are negative regulators of ABA signaling and plant adaptation to stress. Knowledge about the functions of potato group A PP2Cs is limited. Here, we report that the potato group A PP2C StHAB1 is broadly expressed in potato plants and strongly induced by ABA and drought. Suppression of StHAB1 enhanced potato ABA sensitivity and drought tolerance, whereas overexpression of the dominant mutant StHAB1(G276D) compromised ABA sensitivity and drought tolerance. StHAB1 interacts with almost all ABA receptors and the Snf1-Related Kinase OST1. Suppressing StHAB1 and overexpressing StHAB1(G276D) alter potato growth morphology; notably, overexpression of StHAB1(G276D) causes excessive shoot branching. RNA-sequencing analyses identified that the auxin efflux carrier genes StPIN3, StPIN5, and StPIN8 were up-regulated in StHAB1(G276D)-overexpressing axillary buds. Correspondingly, the auxin concentration was reduced in StHAB1(G276D)-overexpressing axillary buds, consistent with the role of auxin in repressing lateral branch outgrowth. The expression of BRANCHED1s (StBRC1a and StBRC1b) was unchanged in StHAB1(G276D)-overexpressing axillary buds, suggesting that StHAB1(G276D) overexpression does not cause axillary bud outgrowth via regulation of BRC1 expression. Our findings demonstrate that StHAB1 is vital in potato drought tolerance and shoot branching.StHAB1, a negative regulator in the abscisic acid signaling pathway, modulates drought tolerance and shoot branching in potato by affecting stomatal opening and auxin transport.