Cytokine profile associated with chronic and acute human schistosomiasis mansoni
Cytokine profile associated with chronic and acute human schistosomiasis mansoni
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DOI：
10.1590/s0074-02762008000600009
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发表时间：
2008-09-01
期刊：
影响因子：
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通讯作者：
Montenegro, Silvia Maria Lucena
中科院分区：
文献类型：
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作者：
Morais, Clarice Neuenschwander Lins de;Souza, Joelma Rodrigues de;Montenegro, Silvia Maria Lucena
对43例不同临床表现的血吸虫病患者血清白细胞介素（IL）、IL-13、IL-4和干扰素-γ（IFN-γ）的产生和调节进行了研究。全血培养细胞因子的生产响应可溶性虫卵抗原（SEA），可溶性蠕虫成虫制剂（SWAP），有丝分裂原，中和抗体或重组IL-13的测定通过ELISA。在SWAP刺激后，慢性患者，特别是肝硬化患者，与急性患者相比产生更高水平的IL-4，表明这些患者中存在2型细胞因子谱。SEA和SWAP刺激后，与急性患者相比，肝脾（HS）患者显示IFN-γ水平升高，表明人类HS疾病与1型细胞因子应答相关。免疫调节的机制在疾病的临床阶段之间明显不同，其中一些是抗原特异性的。
The production and regulation of interleukin (IL) IL-13, IL-4 and interferon-gamma (IFN-gamma) was evaluated in 43 schistosomiasis patients with different clinical forms. Whole-blood cultures cytokine production in response to soluble egg antigen (SEA), soluble worm adult preparation (SWAP), mitogens, neutralizing antibodies or recombinant IL-13 were measured by ELISA. After SWAP stimulation, chronic patients, particularly hepatointestinals, produced higher levels of IL-4 in comparison with acute patients, suggesting the presence of a type 2 cytokine profile in these patients. Following SEA and SWAP stimulation, hepatosplenic (HS) patients showed increased levels of IFN-gamma when compared with acute patients, indicating that HS disease in humans is associated with a type 1 cytokine response. The mechanisms of immune regulation are apparently different between the clinical stages of the disease, some of which are antigen-specific.