Method for the Determination of Ammonia in Mainstream Cigarette Smoke Using Ion Chromatography
Method for the Determination of Ammonia in Mainstream Cigarette Smoke Using Ion Chromatography
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DOI：
10.1371/journal.pone.0159126
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发表时间：
2016-07-14
期刊：
影响因子：
3.7
通讯作者：
Watson, Clifford H.
中科院分区：
文献类型：
--
作者：
Watson, Christina Vaughan;Feng, June;Watson, Clifford H.
主流烟气中的氨以颗粒相和气相两种形式存在。卷烟填料和烟雾中氨的存在具有重要意义，因为氨可能在提高“烟雾 pH 值”方面发挥潜在作用。烟雾 pH 值的增加可能会将总尼古丁的一小部分转化为游离碱尼古丁，据报道，吸烟者可以更快地吸收游离碱尼古丁。测量烟雾中氨的方法通常采用充满酸的撞击器来捕获烟雾。我们开发了一种快速、可靠的方法来测量主流烟雾中的氨，无需使用昂贵且耗时的撞击器来检查氨输送的差异。该方法使用剑桥过滤垫和 Tedlar 袋来捕获烟雾的颗粒物和蒸气相。我们量化了 5 个制造商的 50 个卷烟品牌的主流烟雾中的氨含量。对于 ISO 吸烟条件，每支香烟的氨含量约为 1 微克至 23 微克；对于加拿大强烈吸烟条件，每支香烟的氨含量约为 38 微克至 67 微克，并且在品牌和制造商之间观察到了统计学上的显着差异。我们的研究结果表明，氨水平因品牌而异，并且在加拿大严重吸烟条件下会更高。
Ammonia in mainstream smoke is present in both the particulate and vapor phases. The presence of ammonia in the cigarette filler material and smoke is of significance because of the potential role ammonia could have in raising the "smoke pH." An increased smoke pH could shift a fraction of total nicotine to free-base nicotine, which is reportedly more rapidly absorbed by the smoker. Methods measuring ammonia in smoke typically employ acid filled impingers to trap the smoke. We developed a fast, reliable method to measure ammonia in mainstream smoke without the use of costly and time consuming impingers to examine differences in ammonia delivery. The method uses both a Cambridge filter pad and a Tedlar bag to capture particulate and vapor phases of the smoke. We quantified ammonia levels in the mainstream smoke of 50 cigarette brands from 5 manufacturers. Ammonia levels ranged from approximately 1 mu g to 23 mu g per cigarette for ISO smoking conditions and 38 mu g to 67 mu g per cigarette for Canadian intense smoking conditions and statistically significance differences were observed between brands and manufacturers. Our findings suggest that ammonia levels vary by brand and are higher under Canadian intense smoking conditions.