Optimization of key process variables for enhanced hydrogen production by Enterobacter aerogenes using statistical methods.
Optimization of key process variables for enhanced hydrogen production by Enterobacter aerogenes using statistical methods.
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DOI：
10.1016/j.biortech.2007.04.027
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发表时间：
2008-04
影响因子：
11.4
通讯作者：
J. H. Jo;D. Lee;Donghee Park;W. Choe;J. M. Park
中科院分区：
文献类型：
--
作者：
J. H. Jo;D. Lee;Donghee Park;W. Choe;J. M. Park
在间歇发酵系统中研究了葡萄糖浓度、温度和pH对产气肠杆菌产氢的单独和交互影响。采用Box-Behnken试验设计和响应面分析法（RSM）确定了提高产氢率的最佳条件。通过同时改变对产氢速率有显著影响的三个自变量，考察了产氢速率。在葡萄糖浓度为118.06mM、温度为38°C、pH为6.13的条件下，最大产氢速率为425.8ml H2（g dry cell h）-1。实验结果表明，Box-Behnken设计的响应面方法是获得高产氢速率的有效工具。产气菌
The individual and mutual effects of glucose concentration, temperature and pH on the hydrogen production by Enterobacter aerogenes were investigated in a batch system. A Box–Behnken design and response surface methodology (RSM) were employed to determine the optimum condition for enhanced hydrogen production. The hydrogen production rate was investigated by simultaneously changing the three independent variables, which all had significant influences on the hydrogen production rate. The maximum hydrogen production rate of 425.8ml H2(g dry cellh)−1was obtained under the optimum condition of glucose concentration 118.06mM, temperature 38°C and pH 6.13. The experimental results showed that the RSM with the Box–Behnken design was a useful tool for achieving high rate of hydrogen production by E. aerogenes.