Probing the stability of nonglycosylated wild-type erythropoietin protein via reiterative alanine ligations
Probing the stability of nonglycosylated wild-type erythropoietin protein via reiterative alanine ligations
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DOI：
10.1073/pnas.1202762109
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发表时间：
2012-05-08
影响因子：
11.1
通讯作者：
Danishefsky, Samuel J.
中科院分区：
文献类型：
--
作者：
Brailsford, John A.;Danishefsky, Samuel J.
通过化学方法合成了在半胱氨酸残基上带有乙酰胺基甲基保护基的非糖基化促红细胞生成素。丙氨酸连接用于组装四个肽片段，其本身通过固相肽合成制备。本工作概述了一条合成均相糖基化促红细胞生成素的路线。
Nonglycosylated erythropoietin bearing acetamidomethyl protecting groups at the cysteine residues has been synthesized via chemical methods. Alanine ligation was used to assemble four peptide fragments, themselves prepared by solid phase peptide synthesis. This work outlines a route for the synthesis of homogeneous glycosylated erythropoietin.