Plexin‐B family members demonstrate non‐redundant expression patterns in the developing mouse nervous system: an anatomical basis for morphogenetic effects of Sema4D during development
Plexin‐B family members demonstrate non‐redundant expression patterns in the developing mouse nervous system: an anatomical basis for morphogenetic effects of Sema4D during development
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DOI：
10.1111/j.0953-816x.2004.03401.x
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发表时间：
2004-05
影响因子：
3.4
通讯作者：
T. Worzfeld;A. Püschel;S. Offermanns;R. Kuner
中科院分区：
文献类型：
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作者：
T. Worzfeld;A. Püschel;S. Offermanns;R. Kuner
脑信号蛋白及其受体在发育中的神经系统的连接模式中起着重要作用，最近的数据表明，脑信号蛋白受体的丛蛋白B家族成员可能参与轴突导向。在这里，我们表明，三个丛蛋白-B基因，plxnb 1，plxnb 2和plxnb 3（丛蛋白-B1，丛蛋白-B2和丛蛋白-B3）的mRNA，分别在小鼠发育过程中，在神经系统的外周和中枢成分中以高度特异性和非冗余模式表达。尽管丛蛋白-B1和丛蛋白-B2在神经上皮和发育中的神经元中强烈表达，但丛蛋白-B3 mRNA选择性地定位于白色物质。此外，plexin-B1及其配体Sema 4D在几个区域以互补模式表达，例如发育中的新趾甲皮质，背根神经节和胚胎阶段的脊髓。Sema 4d基因表现出一个戏剧性的开关，从产前表达在神经元群体中的出生后的表达在少突胶质细胞。与其对胚胎视网膜神经节细胞和海马神经元的生长锥的塌陷活性相反，可溶性Sema 4D增强了在胶原基质中培养的胚胎皮质外植体中的轴突生长。因此，丛蛋白B家族成员和Sema 4D可能在神经系统发育过程中发挥复杂和非冗余的作用。
Semaphorins and their receptors play important roles in patterning the connectivity of the developing nervous system and recent data suggest that members of the plexin‐B family of semaphorin receptors may be involved in axonal guidance. Here we show that the mRNAs of the three plexin‐B genes, plxnb1, plxnb2 and plxnb3 (plexin‐B1, plexin‐B2 and plexin‐B3), respectively, are expressed in highly specific and non‐redundant patterns in peripheral and central components of the nervous system over defined periods during murine development. Whereas plexin‐B1 and plexin‐B2 are strongly expressed in the neuroepithelium and developing neurons, plexin‐B3 mRNA is selectively localized to the white matter. Moreover, plexin‐B1 and its ligand Sema4D are expressed in complementary patterns in several regions such as the developing neopallial cortex, the dorsal root ganglia and the spinal cord over embryonic stages. The Sema4d gene demonstrates a dramatic switch from prenatal expression in neuronal populations to a postnatal expression in oligodendrocytes. In contrast to its collapsing activity on growth cones of embryonic retinal ganglion cells and hippocampal neurons, soluble Sema4D enhances axonal outgrowth in embryonic cortical explants cultured in collagen matrices. Thus, plexin‐B family members and Sema4D are likely to play complex and non‐redundant roles during the development of the nervous system.