Estrogen receptor-β in quail:: Cloning, tissue expression and neuroanatomical distribution
Estrogen receptor-β in quail:: Cloning, tissue expression and neuroanatomical distribution
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DOI：
10.1002/(sici)1097-4695(19990905)40:3
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发表时间：
1999-09-05
期刊：
影响因子：
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通讯作者：
Balthazart, J
中科院分区：
文献类型：
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作者：
Foidart, A;Lakaye, B;Balthazart, J
本研究利用3‘-和5’-末端快速扩增技术，对日本鹌鹑雌激素受体-β(ER-beta，ER-β)基因进行了克隆和测序，获得了含有完整开放阅读框架的该基因的全长cDNA序列。这是第一次在鸟类中描述该序列和相应的氨基酸序列，并将它们与以前在哺乳动物中描述的已知ERβ序列以及在一些哺乳动物和禽类物种中鉴定的ERα序列进行比较。Northern blotting分析ERβmRNA在脑和肾脏中的表达，发现存在几个转录本。逆转录聚合酶链式反应证实ERβ的存在显示出广泛的分布，与ERα的分布截然不同。本文还用S-35和P-33标记的寡核苷酸探针进行原位杂交，以确定ERβ基因的完整神经解剖学分布。转录本存在于许多与生殖控制有关的核中，如视前内侧核、终纹核和杏仁核的鸟类同源核带尾核，这些数据表明ERβ在一个非哺乳动物物种中的存在，并表明这种受体的(神经)解剖分布在这两类脊椎动物中是保守的，这种受体在生殖控制和其他生理过程中的作用现在应该被研究。(C)1999 John Wiley&Sons，Inc.J Neurobiol 40：327-342,1999。
A partial estrogen receptor-beta (ER beta) cDNA had been previously cloned and sequenced in Japanese quail, The 3'- and 5'-rapid amplification of cDNA ends techniques were used here to identify a cDNA sequence of the quail ERP that contains a complete open reading frame. For the first time in an avian species, this cDNA sequence and the corresponding amino acid sequence are described, They are compared with the known ER beta sequences previously described in mammals and with the ER alpha sequences identified in a selection of mammalian and avian species. The analysis by Northern blotting of the ER beta mRNA expression in the brain and kidneys revealed the presence of several transcripts. The presence of ER beta identified by reverse transcriptase-polymerase chain reaction demonstrated a widespread distribution quite different from the distribution of ER alpha. The complete neuroanatomical distribution of ER beta mRNA as determined by in situ hybridization with S-35- and P-33-labeled oligoprobes is also presented. Transcripts are present in many nuclei implicated in the control of reproduction such as the medial preoptic nucleus, the nucleus striae terminalis, and the nucleus taeniae, the avian homologue of the amygdala, These data demonstrate the presence of ER beta in a nonmammalian species and indicate that the (neuro)anatomical distribution of this receptor type has been conserved in these two classes of vertebrates, The role of this receptor in the control of reproduction and other physiological processes should now be investigated. (C) 1999 John Wiley & Sons, Inc. J Neurobiol 40: 327-342, 1999.