Kinetic and spectroscopic evidence for an irreversible step between deprotonation and reprotonation of the Schiff base in the bacteriorhodopsin photocycle.
Kinetic and spectroscopic evidence for an irreversible step between deprotonation and reprotonation of the Schiff base in the bacteriorhodopsin photocycle.
复制标题
细菌视紫红质光循环中席夫碱去质子化和再质子化之间不可逆步骤的动力学和光谱证据。
DOI：
10.1021/bi00234a024
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发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Lanyi,JK
中科院分区：
文献类型：
--
作者：
Váró,G;Lanyi,JK
Gyórgy Váró**和Janos K. Lanyi*加州大学生理与生物物理系，加州尔湾92717摘要：采用门控多通道分析仪研究了野生型细菌视紫红质及其D96N形态的光循环。从测量的时间分辨差光谱重建光中间体的光谱，从而评估动力学；pH = 7, 100 mM NaCl存在下的数据最适合用K** L* * MI-* M2** N** 0-* BR + N-*·来拟合
Gyórgy Váró** and Janos K. Lanyi* Department of Physiology and Biophysics, University of California, Irvine, California 92717 Received November 16, 1990; Revised Manuscript Received February 22, 1991 abstract: The photocycles of wild-typebacteriorhodopsin and its D96N form were investigated with a gated multichannel analyzer. Reconstruction of the spectra of the photointermediates from the measured time-resolved difference spectra allowed evaluation of the kinetics; the data at pH 7 in the presence of 100 mM NaCl were best fitted by the scheme K** L*» MI-* M2** N** 0-* BR plus N-*·