Defined anaerobic growth medium for studying Candida albicans basic biology and resistance to eight antifungal drugs
Defined anaerobic growth medium for studying Candida albicans basic biology and resistance to eight antifungal drugs
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DOI：
10.1128/aac.48.7.2350-2354.2004
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发表时间：
2004-07-01
影响因子：
4.9
通讯作者：
Nickerson, KW
中科院分区：
文献类型：
--
作者：
Dumitru, R;Hornby, JM;Nickerson, KW
多态性真菌白色念珠菌是人类中最通用的机会致病菌之一。人体的许多器官都是这种病原体感染的潜在目标，但感染通常局限于胃肠道，这是一种提供厌氧生长条件的环境。我们描述了一种化学成分确定的厌氧生长培养基，用于四种白色念珠菌（A72，SC 5314，MEN和10261）。它是一种补充了油酸、烟酸和氯化铵的确定的液体葡萄糖-磷酸盐-脯氨酸生长培养基。细胞不需要或响应于添加的麦角固醇。油酸和烟酸是仅为C.白色念珠菌本研究的一个重要技术特征是使用厌氧生长的接种物来研究厌氧生长。在厌氧条件下，细胞在25、30和37 ℃下仅以菌丝体形式生长。在30 ℃下的倍增时间约为100 min。20小时这些细胞不产生法尼醇，对外源法尼醇没有反应，并且它们对最高测试水平的阿替霉素B和四种唑类抗真菌剂具有抗性。我们认为C.白念珠菌的拖尾终点现象和耐药可能与白念珠菌的耐药有关。白念珠菌生物膜抗真菌药物。
The polymorphic fungus Candida albicans is one of the most versatile opportunistic pathogens in humans. Many organs of the human body are potential targets for infection by this pathogen, but infection is commonly localized in the gastrointestinal tract, an environment providing anaerobic growth conditions. We describe a chemically defined anaerobic growth medium for four strains of Candida albicans (A72, SC5314, MEN, and 10261). It is a defined liquid glucose-phosphate-proline growth medium supplemented with oleic acid, nicotinic acid, and ammonium chloride. The cells did not require or respond to added ergosterol. Oleic acid and nicotinic acid are growth factors which are required only for the anaerobic growth of C. albicans. An important technical feature of this study was the use of anaerobically grown inocula to study anaerobic growth. Anaerobically, the cells grew exclusively as mycelia at 25, 30, and 37degreesC. The doubling time at 30degreesC was ca. 20 h. The cells did not produce farnesol and did not respond to exogenous farnesol, and they were resistant to the highest tested levels of amphotericin B and four of the azole antifungals. We suggest that the anaerobic growth of C. albicans may contribute to the trailing end point phenomenon and the resistance of C. albicans biofilms to antifungal drugs.