Environmental levels of the antidepressant venlafaxine impact the metabolic capacity of rainbow trout
Environmental levels of the antidepressant venlafaxine impact the metabolic capacity of rainbow trout
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DOI：
10.1016/j.aquatox.2014.06.014
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发表时间：
2014-10-01
影响因子：
4.5
通讯作者：
Vijayan, Mathilakath M.
中科院分区：
文献类型：
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作者：
Best, Carol;Melnyk-Lamont, Nataliya;Vijayan, Mathilakath M.
在经过三级处理的城市污水中，检测到的抗抑郁剂文拉法辛的含量为十亿分之几。然而，这种5-羟色胺-去甲肾上腺素再摄取抑制剂(SNRI)对非靶标水生动物的影响还知之甚少。我们测试了一种假设，即与环境相关的文拉法辛水平扰乱了彩虹鲑鱼(Oncorhynchus MykISS)对压力的高度保守的皮质醇和葡萄糖反应。用文拉法辛(文拉法辛)(0、0.2、1.0mg/L)静态染毒，连续7d。然后，这些鱼受到急性操作干扰，并在应激源暴露后(0h)或1、4和24小时之前进行采样。文拉法辛暴露不影响操控干扰引起的血浆皮质醇水平或靶组织糖皮质激素受体表达的一过性升高。药物暴露扰乱了肾间类固醇合成能力，包括处理应激源介导的类固醇生成急性调节蛋白和细胞色素P450侧链裂解的mRNA丰度的变化。在文拉法辛暴露的鱼中，操作应激源引起的血糖水平的瞬时升高显著减少。肝糖原含量、葡萄糖转运蛋白2mRNA丰度和糖酵解能力无明显变化，而糖异生能力和氨基酸分解代谢能力增强。文拉法辛还引起了鲑鱼鳃的变化，包括乳酸脱氢酶活性和Na+-K+-ATPase蛋白表达增加，而Na+-K+-ATPase酶活性降低。总而言之，我们的结果表明，在水环境中检测到的文拉法辛水平会影响组织代谢能力，并可能损害虹鲑鱼对急性应激源的适应反应。(C)2014爱思唯尔B.V.保留所有权利。
The antidepressant venlafaxine is detected at parts per billion levels in tertiary-treated municipal wastewater effluent. However, the impact of this serotonin-norepinephrine reuptake inhibitor (SNRI) on non-target aquatic animals is poorly understood. We tested the hypothesis that environmentally relevant levels of venlafaxine disrupt the highly conserved cortisol and glucose response to stress in rainbow trout (Oncorhynchus mykiss). Juvenile trout were exposed to venlafaxine (0, 0.2 and 1.0 mu g/L) in a static system with daily renewal for seven days. The fish were then subjected to an acute handling disturbance and sampled either prior to (0 h) or 1,4 and 24h after stressor exposure. Venlafaxine exposure did not affect the handling disturbance-mediated transient elevation in plasma cortisol levels or target tissue glucocorticoid receptor expression. The drug exposure disrupted the interrenal steroidogenic capacity, including altered handling stressor-mediated changes in mRNA abundances of steroidogenic acute regulatory protein and cytochrome P450 side chain cleavage. The handling stressor-induced transient elevations in plasma glucose levels were significantly reduced in the venlafaxine-exposed fish. This was not accompanied by changes in liver glycogen content, glucose transporter 2 mRNA abundance or the glycolytic capacity, whereas the capacity for gluconeogenesis and amino acid catabolism were enhanced. Venlafaxine also brought about changes in the gill of trout, including enhanced lactate dehydrogenase activity and Na+-K+ ATPase protein expression, while the Na+-K+ ATPase enzyme activity was reduced. Collectively, our results demonstrate that venlafaxine at levels detected in the aquatic environment impacts tissue metabolic capacities and may compromise the adaptive responses to an acute stressor in rainbow trout. (C) 2014 Elsevier B.V. All rights reserved.