Thyrotropin-releasing hormone exerts rapid nuclear effects to increase production of the primary prolactin mRNA transcript.
Thyrotropin-releasing hormone exerts rapid nuclear effects to increase production of the primary prolactin mRNA transcript.
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促甲状腺激素释放激素发挥快速核效应，增加初级催乳素 mRNA 转录物的产生。
DOI：
10.1073/pnas.78.11.6662
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发表时间：
1981
影响因子：
11.1
通讯作者：
Rosenfeld,MG
中科院分区：
文献类型：
--
作者：
Potter,E;Nicolaisen,AK;Ong,ES;Evans,RM;Rosenfeld,MG
该报告直接证明了多肽激素可以通过增加初级基因组转录物的水平来调节特定基因的表达。在大鼠垂体瘤细胞系（GH4）中研究了催乳素基因的调控和表达。这些细胞对下丘脑三肽、促甲状腺素释放激素（TRH; thyroliberin）的添加作出反应，通过提高成熟催乳素mRNA的水平和增加催乳素的生物合成。在信息诱导之前，核催乳素RNA前体（包括假定的初级转录物）的快速增加显然是相应的。
This report directly documents that a polypeptide hormone can regulate specific gene expression as a consequence of increasing the levels of a primary genomic transcript. The regulation and expression of the prolactin gene was studied in a cell line (GH4) derived from a rat pituitary tumor. These cells respond to addition of the hypothalamic tripeptide, thyrotropin-releasing hormone (TRH; thyroliberin), by elevation of the levels of mature prolactin mRNA and increase in prolactin biosynthesis. The message induction is preceded by, and apparently consequential to, a comparable rapid increase in the nuclear prolactin RNA precursors, including the putative primary transcript.