Differential effect of adenovirus 2 E3/19K glycoprotein on the expression of H-2Kb and H-2Db class I antigens and H-2Kb- and H-2Db-restricted SV40-specific CTL-mediated lysis.
Differential effect of adenovirus 2 E3/19K glycoprotein on the expression of H-2Kb and H-2Db class I antigens and H-2Kb- and H-2Db-restricted SV40-specific CTL-mediated lysis.
复制标题
腺病毒 2 E3/19K 糖蛋白对 H-2Kb 和 H-2Db I 类抗原的表达以及 H-2Kb 和 H-2Db 限制性 SV40 特异性 CTL 介导的裂解的差异作用。
DOI：
10.1016/0042-6822(88)90580-6
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发表时间：
1988
期刊：
影响因子：
3.7
通讯作者：
Tevethia,SS
中科院分区：
文献类型：
--
作者：
Tanaka,Y;Tevethia,SS
已知由2型腺病毒（Ad 2）编码的E3/19-kDa糖蛋白（E3/19 K）通过与主要组织相容性复合体（MHC）I类抗原细胞内结合来抑制MHC抗原的细胞表面表达，从而减少MHC限制性细胞毒性T淋巴细胞（CTL）对抗原的识别。我们研究了在SV 40感染的猴细胞中E3/19 K的表达，分别表达转染的H-2K和H-2Db抗原的TC-7/H-2K和TC-7/H-2Db对细胞表面H-2 I类抗原的影响，以及对SV 40大肿瘤（T）抗原特异性H-2Kb和H-2Db限制性CTL克隆溶解细胞的影响。在TC-7/H-2Db细胞上，H-2Db抗原的表达在感染Ad 2后12小时即显著降低，而在E3/19 K阴性的SV 40-Ad 2杂合病毒Ad 2 + ND 1感染后12小时则无明显变化。然而，H-2Kb抗原表达的Ad 2感染的TC 7/H-2Kb细胞保持不变，即使在24小时感染后。H-2Db限制性SV 40 T抗原特异性CTL克隆Y-1和Y-3对SV 40感染的TC-7/H-2Db细胞的特异性裂解被Ad 2共感染的靶细胞强烈抑制，但与Ad 2 + ND 1共感染的靶细胞无明显差异。H-2Kb限制的SV 40 T抗原特异性CTL克隆Y-4对SV 40感染的TC-7/H-2Kb细胞的裂解作用也被Ad 2感染显著降低，但不受Ad 2 + ND 1的影响。这些结果表明，E3/19 K蛋白影响H-2Db抗原的细胞表面表达，但不影响H-2Kb抗原。
The E3/19-kDa glycoprotein (E3/19K) coded by adenovirus type 2 (Ad2) is known to inhibit the cell-surface expression of major histocompatibility complex (MHC) class I antigens by binding to the MHC antigens intracellularly, and thus reduces recognition of antigens by MHC-restricted cytotoxic T lymphocytes (CTL). We have studied the effect of the E3/19K expression in SV40-infected monkey cells, TC-7/H-2Kband TC-7/H-2Dbexpressing transfected H-2Kband H-2Dbantigens, respectively, on the cell-surface H-2 class I antigens and on lysis of the cells by SV40 large tumor (T)-antigen-specific H-2Kb- and H-2Db-restricted CTL clones. H-2Dbantigen expression on TC-7/H-2Dbcells was drastically reduced by infection with Ad2 but not with an E3/19K-negative SV40-Ad2 hybrid virus, Ad2+ND1, as early as 12 hr postinfection. However, H-2Kbantigen expression on Ad2-infected TC7/H-2Kbcells remained unaltered, even at 24 hr postinfection. Specific lysis of SV40-infected TC-7/H-2Dbcells by H-2Db-restricted SV40 T-antigen-specific CTL clones, Y-1 and Y-3, was strongly reduced by coinfection of the target cells with Ad2 but not with Ad2+ND1. Lysis of SV40-infected TC-7/H-2Kbcells by a H-2Kb-restricted SV40 T-antigen-specific CTL clone Y-4 was also reduced significantly by Ad2 infection, but not Ad2+ND1. These results indicate that the E3/19K protein affects cell-surface expression of H-2Dbantigen but not H-2Kbantigen.