FREEZE-FRACTURED PURPLE MEMBRANE PARTICLES - PROTEIN-CONTENT

FREEZE-FRACTURED PURPLE MEMBRANE PARTICLES - PROTEIN-CONTENT
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DOI:
10.1126/science.867052
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发表时间:
1977-01-01
期刊:
影响因子:
56.9
通讯作者:
STOECKENIUS, W
STOECKENIUS, W
中科院分区:
综合性期刊1区
文献类型:
--
作者:
FISHER, KA;STOECKENIUS, W

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光学衍射和图像重建可用于将生物膜的EM图像与其电子密度投影相关联。这种相关性表明,[盐生盐杆菌的]单个紫色膜颗粒含有9-12个蛋白质分子,63-84个跨膜α,螺旋,复杂性2-10倍以上,以前建议的膜颗粒。
Optical diffraction and image reconstruction can be used to correlate the EM image of the biological membrane with its electron density projection. Such correlation shows that a single purple membrane particle [of Halobacterium halobium] contains 9-12 protein molecules, 63-84 transmembrane .alpha. helices, a complexity 2-10 times greater than that previously suggested for membrane particles.