Inhibitory Effects of Goishi Tea against Influenza Virus Infection
Inhibitory Effects of Goishi Tea against Influenza Virus Infection
复制标题
五石茶对流感病毒感染的抑制作用
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
H. Yasui
中科院分区：
文献类型：
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作者：
A. Noguchi;Y. Hamauzu;H. Yasui
本研究以日本传统微生物发酵茶--石茶为研究对象，通过体外和体内试验，探讨其抗流感病毒的可能作用。我们发现，Goishi茶热水提取物（GTE）抑制流感A/波多黎各/ 8/ 34（PR 8，H1N1）和流感A/贵州/ 54/ 89（贵州，H3 N2）病毒引起的血凝，PR 8引起的马丹-达比犬肾（MDCK）细胞中的病毒生长，以及鼻接种PR 8引起的小鼠病毒感染。此外，我们研究了GTE的功能组分，发现GTE的高分子量组分抑制病毒吸附到MDCK细胞，低分子量组分抑制感染的后续阶段，如病毒入侵，基因组增殖和释放，吸附后。这些结果表明，GTE通过抑制感染的各个阶段而对流感病毒感染发挥抑制特性。
We investigated the possible anti–influenza virus function of Goishi tea, which is a Japanese traditional microbial fermented tea, in in vitro and in vivo tests. We found that Goishi tea hot water extract (GTE) inhibited hemagglutination caused by influenza A/ Puerto Rico/ 8/ 34 (PR8, H1N1) and influenza A/ Guizhou/ 54/ 89 (Guizhou, H3N2) viruses, viral growth in Mardin-Darby canine kidney (MDCK) cells caused by PR8, and viral infection of mice caused by nasal inoculation of PR8. Furthermore, we investigated the functional fractions of GTE and found that a high-molecular-weight fraction of GTE inhibited viral adsorption to MDCK cells and that low-molecular-weight fractions inhibited subsequent stages of infection, such as viral invasion, genomic multiplication, and release, after adsorption. These results indicate that GTE exerts an inhibitory property against influenza virus infection by inhibiting various stages of infection.