Efferent innervation of the rat testis.
Efferent innervation of the rat testis.
复制标题
大鼠睾丸的传出神经支配。
DOI：
10.1095/biolreprod52.5.1136
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发表时间：
1995
影响因子：
3.6
通讯作者：
Desjardins,C
中科院分区：
文献类型：
--
作者：
Rauchenwald,M;Steers,WD;Desjardins,C
先前对男性生殖道自主神经供应的评估得出的结论是，睾丸主要接收来自主动脉旁神经节，特别是精索神经节的交感神经输入。我们通过使用逆行轴突追踪来检查到达成年大鼠睾丸的传出纤维的来源和分布，从而挑战了这一共识。我们还使用免疫组织化学方法来评估腹部和盆腔神经节睾丸神经元中假定的神经递质。结果表明，大多数逆行标记的细胞分布位于主要骨盆（38％）和盆腔副神经节（37％）内，只有少数标记的细胞分布存在于主动脉旁和精索神经节中。将 FluoroGold 和 Fast Blue 染料分别注射到左右睾丸中表明，当将示踪剂显微注射到对侧睾丸囊下时，盆腔神经节中 17% 的神经元被标记。大约 8% 的神经元同时被标记为 FluoroGold 和 Fast Blue，这表明某些神经元可以同时向左右睾丸提供输入。免疫组织化学结果显示，酪氨酸羟化酶（去甲肾上腺素能纤维的标记物）存在于超过 33% 的用 FluoroGold 或 Fast Blue 标记的细胞谱中。约 27% 的荧光标记细胞谱呈神经肽 Y 阳性，而 22% 的细胞呈血管活性肠多肽免疫反应。睾丸内未检测到血管活性肠多肽免疫反应性的证据，但白膜和睾丸脉管系统中存在神经肽 Y 免疫反应性纤维。儿茶酚胺荧光纤维稀疏地分布在与囊、脉管系统和间质相关的睾丸周围。目前的研究结果提供了新的证据，表明肾上腺素能神经元和假定的去甲肾上腺素能神经元支配大鼠睾丸的囊、脉管系统和周围间质。供应大鼠睾丸的传出神经元起源于盆腔神经节和交感链，而不是像以前根据神经纤维的直接追踪或损伤所推断的精索丛。
Previous assessments of the autonomic nerve supply of the male genital tract concluded that the testis received sympathetic input primarily from paraaortic ganglia, particularly the spermatic ganglion. We challenged this consensus by using retrograde axonal tracing to examine the source and distribution of efferent fibers reaching the testis of adult rats. We also used immunohistochemical methods to assess putative neurotransmitters in testicular neurons of the abdominal and pelvic ganglia. The results indicate that the majority of retrogradely labeled cell profiles were localized within the major pelvic (38%) and pelvic accessory ganglia (37% ), and only a few labeled cell profiles were present in the paraaortic and spermatic ganglia. Injection of FluoroGold and Fast Blue dyes into the respective right and left testis demonstrated that 17% of the neurons in pelvic ganglia were labeled when tracers were microinjected beneath the capsule of the contralateral testis. About 8% of the neurons were labeled both with FluoroGold and Fast Blue, suggesting that certain neurons can provide simultaneous input to the left and right testicles. Immunohistochemical results showed that tyrosine hydroxylase, a marker for noradrenergic fibers, was present in over 33% of the cell profiles labeled with either FluoroGold or Fast Blue. Some 27% of the fluorescent-labeled cell profiles were positive for neuropeptide Y, while 22% were immunoreactive for vasoactive intestinal polypeptide. No evidence for vasoactive intestinal polypeptide immunoreactivity was detected within the testis, but neuropeptide Y-immunoreactive fibers were present in the tunica albuginea and testicular vasculature. Catecholamine fluorescent fibers were distributed sparsely throughout the periphery of the testis in association with the capsule, vasculature, and interstitium. The present findings provide new evidence showing that adrenergic and presumed noradrenergic neurons innervate the capsule, vasculature, and peripheral interstitium of the rat testis. Efferent neurons supplying the rat testis originate in the pelvic ganglia and sympathetic chain, rather than the spermatic plexus as formerly concluded on the basis of direct tracing or lesioning of nerve fibers.