Deep mitochondrial divergence within a Heliconius butterfly species is not explained by cryptic speciation or endosymbiotic bacteria.
Deep mitochondrial divergence within a Heliconius butterfly species is not explained by cryptic speciation or endosymbiotic bacteria.
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DOI：
10.1186/1471-2148-11-358
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发表时间：
2011-12-12
影响因子：
3.4
通讯作者：
Jiggins CD
中科院分区：
文献类型：
--
作者：
Muñoz AG;Baxter SW;Linares M;Jiggins CD
神秘的种群结构可以是早期物种形成或历史过程的指标。我们调查了先前记录的Heliconius erato chestertonii线粒体单倍型中的深断裂，以探索神秘物种形成的可能性，以及可能存在的可能驱动线粒体种群结构的内共生细菌。在来自哥伦比亚西部16个种群的315个个体的样本中，检测到两个主要的mtDNA分支，分歧为2.15%，我们证实了这种结构与地理位置的弱相关。第一个mtDNA分支包含了87%来自北方群体的个体，是H.第二支包含来自南部种群的大部分个体（78%），这些个体与厄瓜多尔的H.埃拉托。AFLP标记分析则显示H. e. chestertonii是一个遗传同质的物种，线粒体分歧和AFLP结构之间没有关联。分子标记之间缺乏一致性表明，神秘的物种形成不是一个合理的解释，在H。和切斯特顿。我们还进行了第一次测试，在Heliconius内共生细菌的存在，并确定了两个不同的谱系内的沃尔巴克氏体H。e.切斯特顿ii。然而，H. e.切斯特顿氏菌与感染模式直接相关。我们的结论是，历史的人口统计学过程是最有可能的解释高线粒体分化的H。e. chestertonii之间的基因流动，可能是由于Cauca valley H. e. chestertonii和西太平洋陆坡种群;埃拉托。
Cryptic population structure can be an indicator of incipient speciation or historical processes. We investigated a previously documented deep break in the mitochondrial haplotypes of Heliconius erato chestertonii to explore the possibility of cryptic speciation, and also the possible presence of endosymbiont bacteria that might drive mitochondrial population structure. Among a sample of 315 individuals from 16 populations of western Colombia, two principal mtDNA clades were detected with 2.15% divergence and we confirmed this structure was weakly associated with geography. The first mtDNA clade included 87% of individuals from northern populations and was the sister group of H. erato members of Andes western, while the second clade contained most individuals from southern populations (78%), which shared haplotypes with an Ecuadorian race of H. erato. In contrast, analysis using AFLP markers showed H. e. chestertonii to be a genetically homogeneous species with no association between mitochondrial divergence and AFLP structure. The lack of congruence between molecular markers suggests that cryptic speciation is not a plausible explanation for the deep mitochondrial divergence in H. e chestertonii. We also carried out the first tests for the presence of endosymbiontic bacteria in Heliconius, and identified two distinct lineages of Wolbachia within H. e. chestertonii. However, neither of the principal mitochondrial clades of H. e. chestertonii was directly associated with the patterns of infection. We conclude that historical demographic processes are the most likely explanation for the high mitochondrial differentiation in H. e. chestertonii, perhaps due to gene flow between Cauca valley H. e. chestertonii and west Pacific slope populations of H. erato.