CENTRAL ACTION OF ACIDIC FIBROBLAST GROWTH-FACTOR IN FEEDING REGULATION
CENTRAL ACTION OF ACIDIC FIBROBLAST GROWTH-FACTOR IN FEEDING REGULATION
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DOI：
10.1152/ajpregu.1989.256.1.r217
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发表时间：
1989-01-01
影响因子：
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通讯作者：
PLATASALAMAN, CR
中科院分区：
文献类型：
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作者：
HANAI, K;OOMURA, Y;PLATASALAMAN, CR
一个阶段性的增加，类似于酸性成纤维细胞生长因子（aFGF）的活性在大鼠的脑脊液中检测到喂养后或腹膜内注射葡萄糖。在两个系统中生物测定脑脊液中的FGF样活性：在肝素亲和柱上分级分离后在BALB/c 3 T3细胞中的Hydra和DNA合成刺激活性的摄食反应的抑制。动态喂养相关的变化，活性的aFGF，碱性FGF，和其他生长因子的生物测定系统检测。侧脑室微量输注aFGF抑制大鼠摄食。以相当于或高于中心给药剂量的灭活aFGF的中心输注或aFGF的外周给药没有效果。电泳应用aFGF特异性抑制下丘脑外侧的葡萄糖敏感神经元的活动。葡萄糖不敏感神经元仅受到轻微影响。提示aFGF可能在中枢神经系统水平参与摄食调节。
A phasic increase in activity similar to that of acidic fibroblast growth factor (aFGF) was detected in the cerebrospinal fluid of rats after feeding or after an intraperitoneal injection of glucose. This FGF-like activity in the cerebrospinal fluid was bioassayed in two systems: depression of the feeding response of Hydra and DNA synthesis-stimulating activity in BALB/c 3T3 cells after fractionation on a heparin affinity column. Dynamic feeding-related changes in activity of aFGF, basic FGF, and other growth factors were detected by both bioassay systems. Intracerebroventricular microinfusion of aFGF suppressed food intake in rats. Central infusion of inactivated aFGF, or peripheral administration of aFGF, in doses equivalent to or higher than those administered centrally was without effect. Electrophoretically applied aFGF specifically suppressed the activity of glucose-sensitive neurons in the lateral hypothalamus. Glucose-insensitive neurons were only slightly affected. The results suggest that aFGF may participate in the regulation of feeding at the level of the central nervous system.