NDRG1 Controls Gastric Cancer Migration and Invasion through Regulating MMP-9
NDRG1 Controls Gastric Cancer Migration and Invasion through Regulating MMP-9
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DOI：
10.1007/s12253-016-0071-8
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发表时间：
2016-10-01
影响因子：
2.8
通讯作者：
Dai, Dongqiu
中科院分区：
文献类型：
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作者：
Chang, Xiaojing;Xu, Xiaoyang;Dai, Dongqiu
本研究旨在探讨NDRG 1在胃癌转移过程中的临床意义及其与MMP-9的关系。采用免疫组化方法检测NDRG 1和MMP-9在101例胃癌组织中的表达，并分析其临床意义。通过siRNA抑制NDRG 1表达，探讨NDRG 1在胃癌发生发展中的作用及其与MMP-9的关系。NDRG 1表达与肿瘤细胞分化程度呈负相关（p < 0.01），浸润深度（p < 0.05），淋巴结转移（p < 0.05）和TNM分期MMP-9与肿瘤细胞分化程度呈正相关（P < 0.05）（p < 0.01）、淋巴结转移（p < 0.05）和TNM分期（p < 0.05），但与浸润深度无关（p > 0.05）。NDRG 1沉默后，细胞增殖和侵袭能力明显增强，但MMP-9表达增加。NDRG 1沉默通过上调MMP-9促进胃癌细胞的生长。NDRG 1可能通过调节MMP-9的表达而抑制胃癌的转移。
The purpose of this study is to detect the clinical significance of NDRG1 and its relationship with MMP-9 in gastric cancer metastatic progression. 101 cases of gastric cancer specimens were utilized to identify the protein expression of NDRG1 and MMP-9 by immunohistochemistry, their clinical significance was also analyzed. The suppression by siRNA-NDRG1 was employed to detect the role of NDRG1 in gastric cancer progression and its relationship with MMP-9. NDRG1 expression was correlated inversely with the degree of tumor cell differentiation (p < 0.01), invasion depth (p < 0.05), lymph node metastasis (p < 0.05) and TNM stage (p < 0.05), whereas MMP-9 was positive correlated with the degree of tumor cell differentiation (p < 0.01), lymph node metastasis (p < 0.05) and TNM stage (p < 0.05), but not correlated with invasion depth (p > 0.05). Furthermore, cell proliferation and invasion effect were remarkably enhanced when NDRG1 was silencing, but MMP-9 expression was increased. NDRG1 silencing enhances gastric cancer cells progression through upregulating MMP-9. It suggests that NDRG1 may inhibit the metastasis of gastric cancer via regulating MMP-9.