Hypoxia enhances proliferation and tissue formation of human mesenchymal stem cells
Hypoxia enhances proliferation and tissue formation of human mesenchymal stem cells
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DOI：
10.1016/j.bbrc.2007.05.054
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发表时间：
2007-07-06
影响因子：
3.1
通讯作者：
Ma, Teng
中科院分区：
文献类型：
--
作者：
Grayson, Warren L.;Zhao, Feng;Ma, Teng
氧浓度的变化影响干细胞和祖细胞的许多先天特性。将人骨髓间充质干细胞（hMSCs）在低氧环境（2%O（2））下培养7代。这导致hMSC在6周内扩增约30倍，而不丧失多谱系分化能力。在缺氧条件下，hMSCs即使在达到融合后也能保持其生长速率，从而形成多层细胞层。缺氧hMSC还表现出细胞和核形态的差异，以及ECM形成和组织的增强。细胞特征的这些变化伴随着Oct-4和HIF-2 α的mRNA水平升高，以及间隙连接蛋白43（一种用于间隙连接形成的蛋白质）表达水平升高。这项研究的结果表明，氧浓度影响干细胞生理学的许多方面，包括生长和体外发育，并且可能是扩增和分化过程中的关键参数。(C)2007爱思唯尔公司All rights reserved.
Changes in oxygen concentrations affect many of the innate characteristics of stem and progenitor cells. Human mesenchymal stem cells (hMSCs) were maintained under hypoxic atmospheres (2% O(2)) for up to seven in vitro passages. This resulted in approximately 30-fold higher hMSC expansion over 6 weeks without loss of multi-lineage differentiation capabilities. Under hypoxia, hMSCs maintained their growth-rates even after reaching confluence, resulting in the formation of multiple cell layers. Hypoxic hMSCs also displayed differences in the cell and nuclear morphologies as well as enhanced ECM formation and organization. These changes in cellular characteristics were accompanied by higher mRNA levels of Oct-4 and HIF-2 alpha, as well as increased expression levels of connexin-43, a protein used in gap junction formation. The results from this study demonstrated that oxygen concentrations affected many aspects of stem-cell physiology, including growth and in vitro development, and may be a critical parameter during expansion and differentiation. (C) 2007 Elsevier Inc. All rights reserved.