Fas-mediated cytotoxicity by freshly isolated natural killer cells.
Fas-mediated cytotoxicity by freshly isolated natural killer cells.
复制标题
DOI：
10.1084/jem.181.3.1235
复制
发表时间：
1995-03-01
期刊：
影响因子：
--
通讯作者：
Saito T
中科院分区：
文献类型：
--
作者：
Arase H;Arase N;Saito T
研究了Fas配体在自然杀伤细胞（NK）上的表达及Fas介导的NK细胞毒性。Fas配体mRNA在新分离的NK细胞中表达，而在T细胞中不表达。此外，通过可溶性Fas分子染色在NK细胞表面检测到Fas配体。我们分析了NK细胞对正常小鼠和lpr小鼠胸腺细胞靶细胞的杀伤活性，发现NK细胞对正常小鼠胸腺细胞有杀伤作用，而对lpr小鼠胸腺细胞无杀伤作用。另一方面，脾T细胞对两种胸腺细胞靶细胞均没有表现出任何细胞毒性。同样，NK细胞对表达Fas抗原的转染物表现出细胞毒性，但对亲本细胞或表达缺失细胞质区突变Fas抗原的转染物没有细胞毒性。这些结果表明NK细胞表达Fas配体，并具有杀伤表面表达Fas抗原的靶细胞的能力。这一发现表明NK细胞在外周淋巴器官中通过组构性或诱导性消除表达fas的细胞发挥重要作用。
The expression of Fas ligand on natural killer (NK) cells and Fas- mediated cytotoxicity by NK cells was investigated. Fas ligand mRNA was expressed in freshly isolated NK cells but not in T cells. Furthermore, the Fas ligand was detected on the cell surface of NK cells by staining with soluble Fas molecule. We analyzed the cytolytic activity of NK cells against thymocyte targets from normal and lpr mice, and found that the NK cells killed thymocytes from normal mice but not from lpr mice. On the other hand, splenic T cells did not show any cytotoxicity against either of the thymocyte targets. Similarly, NK cells exhibited cytotoxicity against transfectants expressing Fas antigen but not against parental cells or transfectants expressing a mutant Fas antigen with deleted cytoplasmic region. These results demonstrated that NK cells express Fas ligand and possess the capability of killing target cells expressing Fas antigen on their surface. This finding suggests that NK cells play an important role by eliminating Fas-expressing cells either constitutively or inducibly in peripheral lymphoid organs.