Heat-shock resistance in experimental cryptorchid testis of mice
Heat-shock resistance in experimental cryptorchid testis of mice
复制标题
DOI：
10.1002/1098-2795(200102)58:2
复制
发表时间：
2001-02-01
影响因子：
2.5
通讯作者：
Endoh, D
中科院分区：
文献类型：
--
作者：
Kon, Y;Endoh, D
Cryptorchidism is commonly used for research on spermatogenesis. However, there are few comparative investigations about the strain differences in mice, especially in long-term experiments. In the present study, the authors demonstrate its specific dynamics in the MRL/MpJ mouse strain, and discuss the cause of strain differences. In the mouse strains A/J BALB/c, C3H/He, and C57BL/6, after 2 weeks of experimental cryptorchidism, the ratios of the cryptorchid testis weight against; the intact one were 0.38 +/- 0.05, 0.43 +/- 0.05, 0.38 +/- 0.02, and 0.44 +/- 0.14, respectively. On the other hand, in the MRL/MpJ strain it was shifted to 0.69 +/- 0.08. The details of this strain difference were compared by calculation of germ cells with the Sertoli cell index at 2 weeks after operation. The indices of spermatogonia in all strains were not significantly different; however, in MRL/MpJ mice remarkable numbers of late spermatocytes and round spermatids were detected. The decrease of the testis weight ratio was similar until 10 days in the C57BL/6 and MRL/MpJ strains, but continued in C57BL/6 until 21 days, whereas in MRL/MpJ mice it plateaued after 10 days. Northern blot analysis for heat shock protein 70-2 using total RNA prepared from the cryptorchid and intact testes at 2 weeks after operation revealed that the expression was decreased in the cryptorchid testis of C57BL/6, but not MRL/MpJ mice. The results suggested that heat-resistant germ cells were present in MRL/MpJ, originating possibly from the genetic background. (C) 2001 Wiley-Liss, Inc.
