The nucleotide sequence of the gal T gene of Escherichia coli.
The nucleotide sequence of the gal T gene of Escherichia coli.
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大肠杆菌gal T基因的核苷酸序列。
DOI：
10.1093/nar/15.19.8116
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发表时间：
1987
影响因子：
14.9
通讯作者：
Frey,PA
中科院分区：
文献类型：
--
作者：
Cornwell,TL;Adhya,SL;Reznikoff,WS;Frey,PA
Lemaire和Muller-Hill最近报道了大肠杆菌gal T基因的核苷酸序列，并推导了半乳糖1-磷酸尿苷酰转移酶（EC 2.7）的氨基酸序列。7.12）（1）。我们通过对EcoRV、Sau 3A、RsaI和PvuII限制性片段应用桑格双脱氧法测定了该基因的核苷酸序列。大约80%的这些序列是在两条链中确定的，而在单链中确定的其余序列被先前发表的部分序列所证实（2-4）。我们获得的序列与Lemaire和Muller-Hill报道的序列相似，除了在核苷酸79和114之间的富含GC的区域。由于广泛的二级结构导致条带压缩，该区域的测序凝胶不明确。我们通过在测序反应中使用dITP代替dGTP克服了模糊性。该程序澄清了测序凝胶，并允许在两条链中测定以下核苷酸序列。与Lemaire和Muller-Hill序列的比较（1）
Lemaire and Muller-Hill recently reported the nucleotide sequence of the gal T gene of Escherichia coli and deduced the amino acid sequence of galactose 1-phosphate uridylyltransferase (EC 2.7. 7.12)(1). We determined the nucleotide sequence of this gene by application of the Sanger dideoxy method to EcoRV, Sau3A, RsaI and PvuII restriction fragments. Approximately 80% of thesequence was determined in both strands, and the remaining sequences determined in single strands were confirmed by partial sequences published earlier (2-4). Thesequence we obtained was similar to that reported by Lemaire and Muller-Hill, with the exception of the GC-rich region between nucleotides 79 and 114. The sequencing gels for this region were ambiguous owing to band compression caused by extensive secondary structure. We overcame the ambiguities by using dITP in place of dGTP in the sequencing reactions. This procedure clarified the sequencing gels and allowed the following nucleotide sequence to be determined in both strands. Comparison with thesequence of Lemaire and Muller-Hill (1)