SALMONELLA INTERACTIONS WITH POLARIZED HUMAN INTESTINAL CACO-2 EPITHELIAL-CELLS
SALMONELLA INTERACTIONS WITH POLARIZED HUMAN INTESTINAL CACO-2 EPITHELIAL-CELLS
复制标题
DOI：
10.1093/infdis/162.5.1096
复制
发表时间：
1990-11-01
影响因子：
6.4
通讯作者：
FALKOW, S
中科院分区：
文献类型：
--
作者：
FINLAY, BB;FALKOW, S
人肠上皮Caco-2细胞系的极化单层细胞在可渗透过滤器上生长，顶端分别感染霍乱沙门氏菌或鼠伤寒沙门氏菌。两种沙门氏菌都能穿透单层，需要2小时才能出现在基底外侧培养基中。这两种细菌在3-4小时内都造成了跨上皮抗性的丧失，在6小时内单层的完整性被完全破坏。扫描和透射电子显微镜显示，细菌与定义明确的顶端微绒毛相互作用，导致刷状边缘的破坏，包括微绒毛的伸长和剥落。细胞质局部也被破坏，有水泡从根尖表面突出。细菌进入（侵入）这些细胞，并被包裹在细胞质内的膜结合液泡中。到6 h时，Caco-2细胞内有许多细菌，这些细菌造成了严重的细胞病变后果。这些形态学观察结果与动物感染模型具有良好的相关性，表明该体外系统将有助于研究与人肠上皮相互作用的病原体。
Polarized monolayers of the human intestinal epithelial Caco-2 cell line were grown on permeable filters and infected apically with either Salmonella choleraesuis or Salmonella typhimurium. Both Salmonella species penetrated through the monolayer, requiring 2 h before appearing in the basolateral medium. Both species caused a loss in transepithelial resistance by 3-4 h, and the monolayer''s integrity was completely disrupted by 6 h. Scanning and transmission electron microscopy revealed that the bacteria interacted with well-defined apical microvilli and caused disruptions in the brush border, including elongation and denuding of the microvilli. The cytoplasm was also disrupted locally, with blebs protruding from the apical surface. The bacterial entered (invaded) these cells and were enclosed in membrane-bound vacuoles within the cytoplasm. By 6 h there were many bacteria within Caco-2 cells, and these organisms caused serious cytopathic consequences. These morphologic observations correlated well with animal infection models, indicating that this in vitro system will be useful to study pathogens that interact with human intestinal epithelia.