Induction of phenoloxidases in the humoral fluids of amphioxus Branchiostoma belcheri by Vibrio alginolyticus and Escherichia coli.
Induction of phenoloxidases in the humoral fluids of amphioxus Branchiostoma belcheri by Vibrio alginolyticus and Escherichia coli.
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DOI：
10.1016/j.fsi.2009.02.019
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发表时间：
2009-04
影响因子：
4.7
通讯作者：
Qiuxiang Pang;Shicui Zhang;Bosheng Zhao
中科院分区：
文献类型：
--
作者：
Qiuxiang Pang;Shicui Zhang;Bosheng Zhao
文昌鱼对微生物攻击的体液免疫反应至今尚未完全阐明。本文研究了文昌鱼体液中PO活性在大肠杆菌和溶藻弧菌攻击前后的变化。结果表明，E.大肠杆菌和溶藻弧菌;并且微生物挑战导致三种PO亚基中的亚基2显著升高，使得PO亚基2成为响应微生物挑战的标记酶。这是首次报道微生物诱导B细胞产生免疫相关分子PO。belcheri。
The humoral immune responses of amphioxus Branchiostoma belcheri to microbial challenge remain open to date. Here we examined the changes in PO activity in the humoral fluids in amphioxus before and after challenge with Escherichia coli and Vibrio alginolyticus. It was found that PO activity in the humoral fluids is markedly increased by challenge with E. coli and V. alginolyticus; and the microbial challenge results in a significant rise in subunit 2 of the three PO subunits, making PO subunit 2 a marker enzyme responsive to microbial challenge. This is the first report on microbial induction of the immune-related molecules like PO in B. belcheri.